Programa de Pós-Graduação em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), 88302-901, Itajaí, SC, Brazil.
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (USP), 14040-903, Ribeirão Preto, SP, Brazil.
Phytother Res. 2017 Oct;31(10):1521-1528. doi: 10.1002/ptr.5876. Epub 2017 Jul 28.
Although Bauhinia forficata Link is popularly used in Brazil to induce diuresis, no scientific investigation has focused on demonstrating its efficacy in preclinical trials. For that, normotensive male Wistar and spontaneously hypertensive rats were used to test the effect of extracts and kaempferitrin obtained from Bauhinia forficata leaves in the experimental model of diuresis. Cumulative urine volume, Na and K excretion, calcium, creatinine, prostaglandin E , pH, density, and conductivity were measured at the end of the experiment (after 8 or 24 h). The treatment with aqueous infusion, methanolic extract, trichloromethane, or ethyl acetate-butanolic fractions significantly increase urinary volume and electrolytes levels when orally given to rats, without altering the pH or density parameters. Kaempferitrin induced diuretic, natriuretic, but not kaliuretic effects in both normotensive and hypertensive rats. In addition, kaempferitrin enhanced urinary creatinine and prostaglandin E excretion, without modifying calcium levels. Kaempferitrin-induced diuresis was unaffected by previous treatment with a nonselective inhibitor of nitric oxide synthase and neither with a nonselective muscarinic receptor antagonist. On the other hand, a cyclooxygenase inhibitor was able to decrease its effect when compared with vehicle-treated rats, suggesting that the diuretic and natriuretic properties from kaempferitrin are associated with endogenous prostanoids generation. Copyright © 2017 John Wiley & Sons, Ltd.
虽然羊蹄甲在巴西被广泛用于利尿，但没有科学研究集中在证明其在临床前试验中的疗效。为此，使用正常血压的雄性 Wistar 大鼠和自发性高血压大鼠来测试从羊蹄甲叶中获得的提取物和山柰酚的实验利尿模型的效果。在实验结束时（8 或 24 小时后）测量累积尿量、Na 和 K 的排泄、钙、肌酐、前列腺素 E、pH 值、密度和电导率。口服给予水提物、甲醇提取物、三氯甲烷或乙酸乙酯-丁醇级分时，可显著增加大鼠的尿量和电解质水平，而不改变 pH 值或密度参数。山柰酚在正常血压和高血压大鼠中均引起利尿、利钠作用，但不引起排钾作用。此外，山柰酚增强了尿肌酐和前列腺素 E 的排泄，而不改变钙水平。一氧化氮合酶非选择性抑制剂和非选择性毒蕈碱受体拮抗剂预先处理均不影响山柰酚诱导的利尿作用。另一方面，与用载体处理的大鼠相比，环氧化酶抑制剂能够降低其作用，表明山柰酚的利尿和利钠特性与内源性前列腺素的产生有关。版权所有 © 2017 约翰威立父子公司
