Nakasone Tasuku, Wakuda Hirokazu, Sugimoto Yumi, Kamei Chiaki
a Department of Pharmacology, Faculty of Pharmaceutical Sciences , Yasuda Women's University , Hiroshima , Japan.
Immunopharmacol Immunotoxicol. 2017 Oct;39(5):292-295. doi: 10.1080/08923973.2017.1354879. Epub 2017 Jul 26.
In this study, we aimed to study the effects of ICI204,448, naloxone methiodide and levocetirizine on the scratching behavior induced by intradermal injection of a ?-opioid antagonist, nor-binaltorphimine (nor-BNI), into the rostral back of ICR mice were investigated.
Male ICR mice weighing 30?35 g were used. The number of scratching episodes were counted for 60 min after i.d. injection of nor-BNI.
nor-BNI dose dependently increased in the number of scratching episodes in ICR mice. nor-BNI-induced scratching behavior was inhibited by not only nalfurafine but also ICI204,448, a peripherally selective ?-opioid agonist. Naloxone and naloxone methiodide, a peripherally restricted ?-opioid antagonist, also inhibited nor-BNI-induced scratching behavior. Scratching behavior induced by nor-BNI was inhibited by chlorpheniramine as well as levocetirizine, a third-generation H antagonist that does not cross into the CNS.
These results suggest that scratching behavior induced by this ?-opioid antagonist, nor-BNI, is related to not only central but also peripheral opioid and H receptors.
在本研究中，我们旨在研究ICI204,448、甲碘化纳洛酮和左西替利嗪对皮内注射μ-阿片受体拮抗剂诺-脑啡肽（nor-BNI）至ICR小鼠鼻背部所诱导的搔抓行为的影响。
使用体重为30-35g的雄性ICR小鼠。皮内注射nor-BNI后60分钟内计数搔抓发作次数。
nor-BNI剂量依赖性增加ICR小鼠的搔抓发作次数。nor-BNI诱导的搔抓行为不仅被纳呋拉啡抑制，还被外周选择性μ-阿片受体激动剂ICI204,448抑制。纳洛酮和外周限制性μ-阿片受体拮抗剂甲碘化纳洛酮也抑制nor-BNI诱导的搔抓行为。nor-BNI诱导的搔抓行为被氯苯那敏以及不进入中枢神经系统的第三代H拮抗剂左西替利嗪抑制。
这些结果表明，这种μ-阿片受体拮抗剂nor-BNI诱导的搔抓行为不仅与中枢阿片受体有关，还与外周阿片受体和H受体有关。
