Faculty of Molecular Chemistry and Engineering, Kyoto Institute of Technology , 1 Hashigami-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
Anal Chem. 2017 Sep 5;89(17):9279-9283. doi: 10.1021/acs.analchem.7b02062. Epub 2017 Aug 8.
This work demonstrates that the solute concentration inside 100 micrometer-sized aqueous microdroplets can be controlled by adjusting the time required for the aqueous nanometer-sized droplets (nanodroplet) or reverse micelles to pass over the surface of the microdroplet. The kinetics of molecular transport between the microdroplets and the nanodroplets was investigated by utilizing a microdroplet array, and on the basis of these results, a control over the concentration selectivity of the contents of the microdroplet was achieved. This method is operationally simple and can be potentially applied as a pretreatment method for microanalytical systems that require high-density microdroplet arrays. This method can also be utilized for parallel small sample analyses such as single cell analysis.
这项工作表明，可以通过调整纳米级水液滴（纳米液滴）或反胶束通过微液滴表面所需的时间来控制 100 微米大小的水微液滴内的溶质浓度。通过利用微液滴阵列研究了微液滴与纳米液滴之间分子传输的动力学，在此基础上，实现了对微液滴内容物浓度选择性的控制。该方法操作简单，可作为需要高密度微液滴阵列的微分析系统的预处理方法。该方法还可用于单细胞分析等并行小样本分析。
