Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba, Sendai, Miyagi, 980-8577, Japan.
Department of Chemistry, Graduate School of Science, Tohoku University, 6-3 Aramaki, Aoba, Sendai, Miyagi, 980-8577, Japan.
Anal Sci. 2021 May 10;37(5):753-758. doi: 10.2116/analsci.20SCP18. Epub 2021 Jan 22.
The partitioning of water and tetramethylrhodamine-conjugated-10-residue oligopeptides from the aqueous phase of microdroplets into Span 80 reverse micelles was observed by utilizing microdroplet arrays. Each peptide was dissolved in phosphate buffer saline, and initially encapsulated in arrayed droplets. An organic phase containing the reverse micelles was added to the microdroplets. Here, the hydration degree of the reverse micelle was adjusted by contact of the organic phase with a 1.0 M NaCl aqueous solution or with a phosphate buffer saline before combining it with the microdroplets. For micelles treated with a 1.0 M NaCl, significant water transport from the microdroplet to the micelle was observed, and peptide with low solubility in water was transported to the reverse micelles, while those with high solubility in water were not. For micelles treated with phosphate buffer saline, the water transport was minimal, and no significant peptide transport was observed. These results suggest that the partitioning of low-solubility oligopeptides requires accompanying water transport to the reverse micelle phase.
利用微滴阵列观察到水和四甲基罗丹明-10 残基寡肽从微滴的水相分配到司盘 80 反胶束中。每个肽都溶解在磷酸盐缓冲盐水中，并最初封装在阵列化的液滴中。含有反胶束的有机相被添加到微滴中。在这里，通过有机相与 1.0 M NaCl 水溶液或磷酸盐缓冲盐水接触来调节反胶束的水合程度，然后再将其与微滴结合。对于用 1.0 M NaCl 处理的胶束，观察到从微滴到胶束的显著水传输，并且在水中低溶解度的肽被运输到反胶束中，而在水中高溶解度的肽则没有。对于用磷酸盐缓冲盐水处理的胶束，水传输最小，并且没有观察到显著的肽传输。这些结果表明，低溶解度寡肽的分配需要伴随水向反胶束相的传输。
