Department of Obstetrics and Gynecology, Zaozhuang Muicipal Hospital, Zaozhuang, Shandong Province, China.
Eur Rev Med Pharmacol Sci. 2017 Jul;21(3 Suppl):12-19.
To study the apoptotic signaling pathways of recurrent spontaneous abortion caused by dysfunction of trophoblast infiltration.
60 patients with recurrent spontaneous abortion and normal abortion were selected consecutively as recurrent spontaneous abortion group and abortion group, respectively. Villous tissues were obtained and cell apoptosis was observed under a microscope; terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (Tunel) method was used to test the apoptosis rate. In situ hybridization was adopted to detect expressions of Fas messenger RNA (Fas mRNA) and Fas ligand messenger RNA (FasL mRNA); expression of Fas, FasL and protein kinase C (PKC) were examined by immunohistochemistry at protein level; fluorescence spectrophotometer was used to test Ca2+ level.
The apoptosis rate, expressions of Fas mRNA, and FasL mRNA, expressions of Fas and FasL proteins, as well as Ca2+ level, were significantly higher in the recurrent spontaneous abortion group than in abortion group. The level of PKC protein was significantly lower in recurrent spontaneous abortion group than in abortion group (p<0.05).
Fas-FasL and PKC signaling pathways, as well as Ca2+, may mediate the dysfunction of trophoblast infiltration, which leads to recurrent spontaneous abortion.
研究胎盘滋养细胞浸润功能障碍导致复发性自然流产的细胞凋亡信号通路。
连续选择 60 例复发性自然流产患者和正常流产患者作为复发性自然流产组和流产组。观察显微镜下绒毛组织细胞凋亡情况；采用末端脱氧核苷酸转移酶（TdT）介导的 dUTP 缺口末端标记（Tunel）法检测细胞凋亡率。原位杂交法检测 Fas 信使 RNA（Fas mRNA）和 Fas 配体信使 RNA（FasL mRNA）的表达；采用免疫组织化学法检测 Fas、FasL 和蛋白激酶 C（PKC）的蛋白表达；荧光分光光度计检测 Ca2+水平。
复发性自然流产组的细胞凋亡率、Fas mRNA 和 FasL mRNA 的表达、Fas 和 FasL 蛋白的表达以及 Ca2+水平均显著高于流产组。复发性自然流产组的 PKC 蛋白水平显著低于流产组（p<0.05）。
Fas-FasL 和 PKC 信号通路以及 Ca2+可能介导胎盘滋养细胞浸润功能障碍，导致复发性自然流产。
