Nayak B N, Buttar H S
Teratog Carcinog Mutagen. 1986;6(2):83-91. doi: 10.1002/tcm.1770060202.
The chromosome-damaging potential of gossypol was evaluated by scoring sister chromatid exchanges (SCEs), determining the percentage of pulverized metaphases and the mitotic index in bone marrow cells of mice. Bone marrow cells were collected approximately 21 hours after the intraperitoneal (0,20,40,80, or 160 micrograms/g) and oral (0,40,80, or 160 micrograms/g) administration of gossypol acetic acid. Irrespective of the dosing schedule (single or multiple doses), the vehicle used (physiological saline, corn oil, or 10% aqueous ethanol), and the route of administration, the mean SCE count per cell was significantly higher (P less than 0.05) in gossypol-treated groups than their control counterparts. At 80 and 160 micrograms/g dose levels, the occurrence of metaphase chromosome pulverization was significantly greater, while mitotic index values were markedly lower than those of the corresponding control values. The results suggest that gossypol is a potentially mutagenic and clastogenic agent in murine bone marrow cells.
通过对小鼠骨髓细胞中的姐妹染色单体交换（SCE）进行评分、确定粉碎中期的百分比以及有丝分裂指数，来评估棉酚对染色体的损伤潜力。在腹腔注射（0、20、40、80或160微克/克）和口服（0、40、80或160微克/克）醋酸棉酚约21小时后，收集骨髓细胞。无论给药方案（单剂量或多剂量）、所用赋形剂（生理盐水、玉米油或10%乙醇水溶液）以及给药途径如何，棉酚处理组每细胞的平均SCE计数均显著高于相应对照组（P<0.05）。在80和160微克/克剂量水平下，中期染色体粉碎的发生率显著更高，而有丝分裂指数值明显低于相应的对照值。结果表明，棉酚在小鼠骨髓细胞中是一种潜在的诱变剂和染色体断裂剂。
