Buttar H S, Nayak B N
Bureau of Drug Research, Health Protection Branch, Health and Welfare Canada, Sir Frederick Banting Research Centre, Tunney's Pasture, Ottawa, Ont.
Toxicol Lett. 1987 Oct;38(3):251-6. doi: 10.1016/0378-4274(87)90005-1.
The cytogenetic effects of gossypol were evaluated by determining the frequency of sister chromatid exchanges (SCEs), percentage of pulverized metaphases, mitotic indices and micronuclei in bone marrow cells of mice treated per vaginam. A dose-dependent increase in the frequency of SCEs was observed when gossypol suspended in corn oil was administered at dosages of 10, 20 or 40 micrograms/g. In comparison with controls, incidences of SCEs were significantly higher in mice given 20 and 40 micrograms/g gossypol, whereas the mitotic indices, percentages of pulverized metaphases and the frequency of interphase micronuclei in treated animals were not different from their control counterparts. The SCE data suggest that gossypol has a DNA-damaging potency in murine bone marrow cells.
通过测定经阴道给药的小鼠骨髓细胞中姐妹染色单体交换（SCE）频率、粉碎中期百分比、有丝分裂指数和微核，评估棉酚的细胞遗传学效应。当以10、20或40微克/克的剂量给予悬浮于玉米油中的棉酚时，观察到SCE频率呈剂量依赖性增加。与对照组相比，给予20和40微克/克棉酚的小鼠中SCE发生率显著更高，而处理组动物的有丝分裂指数、粉碎中期百分比和间期微核频率与对照动物无差异。SCE数据表明棉酚对小鼠骨髓细胞具有DNA损伤潜能。
