Kurogi Katsuhisa, Sakakibara Yoichi, Suiko Masahito, Liu Ming-Cheh
Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo Health Science Campus, OH, USA.
Department of Biochemistry and Applied Biosciences, University of Miyazaki, Japan.
FEBS Lett. 2017 Aug;591(16):2417-2425. doi: 10.1002/1873-3468.12767. Epub 2017 Aug 9.
While 25-hydroxyvitamin D 3-O-sulfate is known to be present in circulation, how it is generated in the body remains unclear. This study aimed to investigate its sulfation in major human organs and to unveil the responsible cytosolic sulfotransferases (SULTs). Of the vitamin D -related compounds tested, 25-hydroxyvitamin D and 7-dehydrocholesterol are preferentially sulfated by human organ cytosols. Among the 13 human SULTs, SULT2A1 shows sulfating activity toward all vitamin D -related compounds, whereas SULT1A1 and SULT2B1a/SULT2B1b show sulfating activity exclusively for, respectively, calcitriol and 7-dehydrocholesterol. These findings suggest that the metabolic pathway leading to the formation of 25-hydroxyvitamin D 3-O-sulfate may be mediated by the sulfation of 25-hydroxyvitamin D or by the conversion of 7-dehydrocholesterol-3-O-sulfate in the skin.
虽然已知25-羟基维生素D 3-O-硫酸盐存在于血液循环中，但其在体内的生成方式仍不清楚。本研究旨在调查其在人体主要器官中的硫酸化作用，并揭示相关的胞质硫酸转移酶（SULTs）。在所测试的维生素D相关化合物中，25-羟基维生素D和7-脱氢胆固醇优先被人体器官胞质硫酸化。在13种人类SULTs中，SULT2A1对所有维生素D相关化合物均表现出硫酸化活性，而SULT1A1和SULT2B1a/SULT2B1b分别仅对骨化三醇和7-脱氢胆固醇表现出硫酸化活性。这些发现表明，导致25-羟基维生素D 3-O-硫酸盐形成的代谢途径可能是由25-羟基维生素D的硫酸化或皮肤中7-脱氢胆固醇-3-O-硫酸盐的转化介导的。
