Shahhosseini Nariman, Chinikar Sadegh, Moosa-Kazemi Seyed Hassan, Sedaghat Mohammad Mehdi, Kayedi Mohammad Hassan, Lühken Renke, Schmidt-Chanasit Jonas
Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, Hamburg, Germany.
Pasteur Institute of Tehran, Tehran, Iran.
Trop Med Int Health. 2017 Oct;22(10):1343-1349. doi: 10.1111/tmi.12935. Epub 2017 Aug 23.
Screening of mosquitoes for viruses is an important forecasting tool for emerging and re-emerging arboviruses. Iran has been known to harbour medically important arboviruses such as West Nile virus (WNV) and dengue virus (DENV) based on seroepidemiological data. However, there are no data about the potential mosquito vectors for arboviruses in Iran. This study was performed to provide mosquito and arbovirus data from Iran.
A total of 32 317 mosquitos were collected at 16 sites in five provinces of Iran in 2015 and 2016. RT-PCR for detection of flaviviruses was performed. The PCR amplicons were sequenced, and 109 WNV sequences, including one obtained in this study, were used for phylogenetic analyses.
The 32 317 mosquito specimens belonging to 25 species were morphologically distinguished and distributed into 1222 pools. Culex pipiens s.l. comprised 56.429%. One mosquito pool (0.08%), containing 46 unfed Cx. pipiens pipiens form pipiens (Cpp) captured in August 2015, was positive for flavivirus RNA. Subsequent sequencing and phylogenetic analyses revealed that the detected Iranian WNV strain belongs to lineage 2 and clusters with a strain recently detected in humans. No flaviviruses other than WNV were detected in the mosquito pools.
Cpp could be a vector for WNV in Iran. Our findings indicate recent circulation of WNV lineage-2 strain in Iran and provide a solid base for more targeted arbovirus surveillance programs.
对蚊子进行病毒筛查是预测新出现和再次出现的虫媒病毒的重要工具。根据血清流行病学数据，伊朗已知携带西尼罗河病毒（WNV）和登革热病毒（DENV）等具有医学重要性的虫媒病毒。然而，尚无关于伊朗虫媒病毒潜在蚊媒的数据。本研究旨在提供来自伊朗的蚊子和虫媒病毒数据。
2015年和2016年在伊朗五个省份的16个地点共采集了32317只蚊子。进行了用于检测黄病毒的逆转录聚合酶链反应（RT-PCR）。对PCR扩增产物进行测序，并将109条WNV序列（包括本研究获得的一条序列）用于系统发育分析。
形态学上区分出属于25个物种的32317只蚊子标本，并将其分成1222个池。尖音库蚊复合组占56.429%。一个蚊子池（0.08%），包含2015年8月捕获的46只未进食的尖音库蚊指名亚种（Cpp），黄病毒RNA检测呈阳性。随后的测序和系统发育分析表明，检测到的伊朗WNV毒株属于2型谱系，与最近在人类中检测到的一个毒株聚类。在蚊子池中未检测到除WNV以外的其他黄病毒。
Cpp可能是伊朗WNV的传播媒介。我们的研究结果表明WNV 2型谱系毒株最近在伊朗传播，并为更有针对性的虫媒病毒监测计划提供了坚实基础。
