M'ghirbi Youmna, Mousson Laurence, Moutailler Sara, Lecollinet Sylvie, Amaral Rayane, Beck Cécile, Aounallah Hajer, Amara Meriem, Chabchoub Ahmed, Rhim Adel, Failloux Anna-Bella, Bouattour Ali
Laboratoire Des Virus, Vecteurs et Hôtes (LR20IPT02), Institut Pasteur de Tunis, Université Tunis El Manar, Tunis 1002, Tunisia.
Institut Pasteur, Department of Virology, Arboviruses and Insect Vectors, 25-28 Rue du Docteur Roux, 75724 Paris, France.
Pathogens. 2023 Feb 21;12(3):360. doi: 10.3390/pathogens12030360.
Mosquito-borne diseases have a significant impact on humans and animals and this impact is exacerbated by environmental changes. However, in Tunisia, surveillance of the West Nile virus (WNV) is based solely on the surveillance of human neuroinvasive infections and no study has reported mosquito-borne viruses (MBVs), nor has there been any thorough serological investigation of anti-MBV antibodies in horses. This study therefore sought to investigate the presence of MBVs in Tunisia. Among tested mosquito pools, infections by WNV, Usutu virus (USUV), and Sindbis virus (SINV) were identified in . The serosurvey showed that 146 of 369 surveyed horses were positive for flavivirus antibodies using the cELISA test. The microsphere immunoassay (MIA) showed that 74 of 104 flavivirus cELISA-positive horses were positive for WNV, 8 were positive for USUV, 7 were positive for undetermined flaviviruses, and 2 were positive for tick-borne encephalitis virus (TBEV). Virus neutralization tests and MIA results correlated well. This study is the first to report the detection of WNV, USUV and SINV in in Tunisia. Besides, it has shown that there is a significant circulation of WNV and USUV among horses, which is likely to cause future sporadic outbreaks. An integrated arbovirus surveillance system that includes entomological surveillance as an early alert system is of major epidemiological importance.
蚊媒疾病对人类和动物有重大影响，而环境变化加剧了这种影响。然而，在突尼斯，西尼罗河病毒（WNV）的监测仅基于对人类神经侵袭性感染的监测，尚无研究报告蚊媒病毒（MBV），也没有对马匹中的抗MBV抗体进行任何全面的血清学调查。因此，本研究旨在调查突尼斯MBV的存在情况。在检测的蚊群中，发现了西尼罗河病毒、乌苏图病毒（USUV）和辛德毕斯病毒（SINV）感染。血清学调查显示，使用cELISA检测，369匹被调查马匹中有146匹黄病毒抗体呈阳性。微球免疫测定（MIA）显示，104匹黄病毒cELISA阳性马匹中有74匹西尼罗河病毒呈阳性，8匹乌苏图病毒呈阳性，7匹未确定的黄病毒呈阳性，2匹蜱传脑炎病毒（TBEV）呈阳性。病毒中和试验和MIA结果相关性良好。本研究首次报告在突尼斯检测到西尼罗河病毒、乌苏图病毒和辛德毕斯病毒。此外，研究表明西尼罗河病毒和乌苏图病毒在马匹中大量传播，这可能导致未来的零星疫情爆发。一个包括昆虫学监测作为早期预警系统的综合虫媒病毒监测系统具有重要的流行病学意义。
