Ding Chao-Qun, Li Kun-Rong, Duan Yun-Xia, Jia Shi-Ru, Lv He-Xin, Bai He, Zhong Cheng
Key Laboratory of Industrial Fermentation Microbiology, (Ministry of Education), Tianjin University of Science & Technology, Tianjin, 300457 People's Republic of China.
Tianjin Academy of Environmental Sciences, Tianjin, 300191 China.
Bioresour Bioprocess. 2017;4(1):31. doi: 10.1186/s40643-017-0159-3. Epub 2017 Jul 10.
Enrichment culture was applied to obtain microbial consortium from activated sludge samples collected from biodegradation system, a chemical fiber plant in Hebei Province, China. Bacterial composition and community dynamic variation were assessed employing denaturing gradient gel electrophoresis fingerprinting technology based on amplified 16S rRNA genes in the entire process of enrichment culture for viscose fiber wastewater.
Four bacteria named as VF01, VF02, VF03, and VF04 were isolated from the microbial consortium adopting the spray-plate method. The DNA bands of these four bacteria were corresponded to the predominant DNA bands in the electrophoresis pattern. VF01, VF02, VF03, and VF04 were phylogenetically closed to , , , and sp. by sequence analysis, respectively. The degradation effects for COD of single isolated strain, mixed strains, and microbial consortium (VF) originally screened from viscose fiber wastewater were determined. The degradation ability was as follows: microbial consortium (VF) > mixed strains > single isolated strain. Microbial consortium (VF) showed the optimum degradation rate of COD of 87% on 14th day. Degradation of pollutants sped up by bio-augmentation of four strains. The molecular weight distribution of organic matter showed that viscose fiber wastewater contained a certain amount of large molecular organic matter, which could be decomposed into smaller molecular substances by microbial consortium (VF).
The microbial consortium (VF) obtained from enrichment culture exhibited great potential for COD degradation. The screened strains had bio-augmentation functions and the addition of a mixture of four bacteria could speed up the degradation rate of pollutants.
采用富集培养法从中国河北省某化纤厂生物降解系统采集的活性污泥样品中获取微生物群落。在粘胶纤维废水富集培养的全过程中，基于扩增的16S rRNA基因，采用变性梯度凝胶电泳指纹技术评估细菌组成和群落动态变化。
采用喷雾平板法从微生物群落中分离出4株细菌，分别命名为VF01、VF02、VF03和VF04。这4株细菌的DNA条带与电泳图谱中的主要DNA条带相对应。通过序列分析，VF01、VF02、VF03和VF04在系统发育上分别与 、 、 和 属的菌株相近。测定了从粘胶纤维废水中最初筛选出的单株分离菌、混合菌株和微生物群落（VF）对化学需氧量（COD）的降解效果。降解能力如下：微生物群落（VF）＞混合菌株＞单株分离菌。微生物群落（VF）在第14天显示出最佳的COD降解率，为87%。4株菌株的生物强化作用加快了污染物的降解。有机物的分子量分布表明，粘胶纤维废水中含有一定量的大分子有机物，这些有机物可被微生物群落（VF）分解为较小分子的物质。
通过富集培养获得的微生物群落（VF）对COD降解具有巨大潜力。筛选出的菌株具有生物强化功能，添加4种细菌的混合物可加快污染物的降解速率。
