College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, PR China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, PR China.
College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, PR China.
Bioresour Technol. 2015 Aug;190:381-7. doi: 10.1016/j.biortech.2015.04.105. Epub 2015 May 1.
In this work, spent mushroom substrates were utilized for screening a microbial consortium with highly simultaneous degradation of lignocellulose and chlorophenols. The desired microbial consortium OEM1 was gained through successive cultivation for about 50 generations and its stability of composition was verified by denaturing gradient gel electrophoresis (DGGE) during screening process. It could degrade lignocellulose and chlorophenols at around 50% and 100%, respectively, within 7days. The diversity analysis and the growth characteristics of OEM1 during degradation process were investigated by PCR-DGGE combined with clone and sequence. The results indicated that OEM1 consisted of 31 strains. Proteobacteria and Bacteroidetes were the predominant bacterial groups. The dynamic change of OEM1 illustrated that consortium community structure was effected by pH and substrate alteration and tended to be stable after 6days' cultivation. Furthermore, bacteria (11 strains) and actinomycetes (2 strains) were obtained based on plate isolation and identified via 16S rDNA sequence.
在这项工作中，利用废弃的蘑菇基质筛选出了一个具有高效协同降解木质纤维素和氯酚能力的微生物群落。通过大约 50 代的连续培养，获得了理想的微生物群落 OEM1，并且在筛选过程中通过变性梯度凝胶电泳（DGGE）验证了其组成的稳定性。OEM1 可以在 7 天内分别将木质纤维素和氯酚降解约 50%和 100%。通过 PCR-DGGE 结合克隆和测序对 OEM1 在降解过程中的多样性分析和生长特性进行了研究。结果表明，OEM1 由 31 株菌组成。变形菌门和拟杆菌门是主要的细菌群体。OEM1 的动态变化表明，群落结构受到 pH 和基质变化的影响，在 6 天的培养后趋于稳定。此外，通过平板分离获得了 11 株细菌（11 株）和 2 株放线菌，并通过 16S rDNA 序列进行了鉴定。
