Antiplasmodial activity and cytotoxicity of ethanol extract of root.

OBJECTIVE

root decoction that has been traditionally used for the treatment of malaria by various tribes in Nigeria, was evaluated for antimalarial potential against malaria parasites using and models.

MATERIALS AND METHODS

The root extract of was investigated for antimalarial activity against in mice using rodent malaria models; suppressive, prophylactic and curative tests and antiplasmodial activity against chloroquine-sensitive (Pf 3D7) and resistant (Pf INDO) strains of using SYBR green assay method. Median lethal dose and cytotoxic activity against HeLa and HEKS cells were assessed and phytochemical screening was also carried out using standard procedures.

RESULTS

The LD value of root extract was found to be 474.34 mg/kg. The crude extract (45-135 mg/kg, p.o) showed significant (p<0.05-0.001) antimalarial activity against infection in suppressive, prophylactic and curative tests with a prolonged survival time. The crude extract also showed moderate activity against both chloroquine-sensitive (Pf 3D7) and resistant (Pf INDO) strains of with an IC value of 71.62±3.38 μg/ml (for Pf 3D7) and 63.76±4.12 μg/ml (for Pf INDO). The crude extract was not cytotoxic to the two cell lines tested with TC of >100 μg/ml against both HeLa and HEKS cell lines.

CONCLUSION

These results suggest that the root extract of possesses antimalarial activity against both chloroquine-sensitive and resistant malaria and these data justify its use in ethnomedicine to treat malaria infections.