Agriculture College, Yanbian University, Yanji, 133000, China.
College of Agriculture and Forestry Science, Linyi University, Shuangling Road, Linyi, 276005, China.
Transgenic Res. 2017 Oct;26(5):677-687. doi: 10.1007/s11248-017-0036-z. Epub 2017 Jul 26.
Producing aflatoxin-detoxifizyme (ADTZ) in pigs to control the AFT contamination of pig feed is a new research strategy by transgenic technology. In this study, transgenic pigs specifically expressing ADTZ gene in the parotid gland were successfully produced by somatic cell nuclear transfer technology. The ADTZ activity in saliva of 6 transgenic pigs was found to be 7.11 ± 2.63 U/mL. The feeding trial with aflatoxin (AFT) results showed that there were significant difference about the serum biochemical index such as total protein (TP), albumin (ALB), globulin (GLB) contents and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity and AFT residues in serum and liver between the pigs in the test treatment (transgenic pigs) producing ADTZ and those in the positive control (P < 0.05). In order to investigate the inheritance of the transgene, 11 G1 transgenic pigs were successfully obtained. The ADTZ activity in saliva of 11 G1 transgenic pigs was found to be 5.82 ± 1.53 U/mL. The feeding trial with AFT results showed that the serum biochemical index containing TP, ALB and GLB contents and ALT and AST activity and AFB1 residues in serum and liver of the pigs in the test treatment (transgenic pigs) producing ADTZ were significantly different than those in the positive control (P < 0.05). The above results demonstrated that ADTZ produced in transgenic pigs could improve the effect of the AFT contamination of feed on pigs.
利用转基因技术在猪中生产黄曲霉毒素解毒酶（ADTZ）以控制猪饲料中 AFT 的污染是一种新的研究策略。在本研究中，通过体细胞核移植技术成功生产了特异性在腮腺中表达 ADTZ 基因的转基因猪。发现 6 头转基因猪唾液中的 ADTZ 活性为 7.11±2.63 U/mL。黄曲霉毒素（AFT）的喂养试验结果表明，实验组（产生 ADTZ 的转基因猪）和阳性对照组（P<0.05）的血清生化指标如总蛋白（TP）、白蛋白（ALB）、球蛋白（GLB）含量和丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）活性以及血清和肝脏中的 AFT 残留量存在显著差异。为了研究转基因的遗传情况，成功获得了 11 头 G1 代转基因猪。发现 11 头 G1 代转基因猪唾液中的 ADTZ 活性为 5.82±1.53 U/mL。AFT 的喂养试验结果表明，实验组（产生 ADTZ 的转基因猪）的血清生化指标如 TP、ALB 和 GLB 含量以及 ALT 和 AST 活性以及血清和肝脏中的 AFB1 残留量与阳性对照组（P<0.05）存在显著差异。上述结果表明，转基因猪产生的 ADTZ 可以提高饲料中 AFT 污染对猪的影响效果。
