CSIRO Health and Biosecurity, Geelong, VIC, 3219, Australia.
CSIRO Agriculture and Food, Werribee, VIC, 3030, Australia.
Transgenic Res. 2019 Feb;28(1):51-76. doi: 10.1007/s11248-018-0101-2. Epub 2018 Oct 29.
Zoonotic and foodborne diseases pose a significant burden, decreasing both human and animal health. Modifying chickens to overexpress antimicrobials has the potential to decrease bacterial growth on poultry products and boost chicken innate immunity. Chickens overexpressing either ovotransferrin or avian β-defensin-3 (AvβD3) were generated using Tol-2 transposons. Transgene expression at the RNA and protein level was seen in egg white, breast muscle, and serum. There were significant differences in the immune cell populations in the blood, bursa, and spleen associated with transgene expression including an increased proportion of CD8+ cells in the blood of ovotransferrin and AvβD3 transgenic birds. Expression of the antimicrobials inhibited the in vitro growth of human and chicken bacterial pathogens and spoilage bacteria. For example, transgene expression significantly reduced growth of aerobic and coliform bacteria in breast muscle and decreased the growth of Salmonella enterica in egg white. Overall these results indicate that overexpression of antimicrobials in the chicken can impact the immune system and increase the antimicrobial capacity of poultry products.
人畜共患病和食源性病原体对人类和动物健康造成了严重负担。通过过表达抗菌肽来修饰鸡，可以减少禽产品中的细菌生长，增强鸡的固有免疫力。使用 Tol-2 转座子生成了过表达卵转铁蛋白或禽 β-防御素-3（AvβD3）的鸡。在蛋清、胸肌和血清中观察到 RNA 和蛋白质水平的转基因表达。与转基因表达相关的血液、腔上囊和脾脏中的免疫细胞群存在显著差异，包括卵转铁蛋白和 AvβD3 转基因鸡血液中 CD8+细胞比例增加。抗菌肽的表达抑制了人类和鸡源性细菌病原体和腐败细菌的体外生长。例如，转基因表达显著降低了胸肌中需氧菌和大肠菌群的生长，并降低了蛋清中肠炎沙门氏菌的生长。总的来说，这些结果表明，鸡中抗菌肽的过表达可以影响免疫系统并增加家禽产品的抗菌能力。
