Gajger Ivana Tlak, Sakač Martina, Gregorc Aleš
Laboratory for Honeybee Diseases - NRL, Department for Biology and Pathology of Fish and Bees, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000, Zagreb, Croatia.
Agricultural Institute of Slovenia, Hacquetova 17, 1000, Ljubljana, Slovenia.
Bull Environ Contam Toxicol. 2017 Sep;99(3):297-302. doi: 10.1007/s00128-017-2144-0. Epub 2017 Jul 26.
High honey bee losses around the world have been linked in part by the regular use of neonicotinoids in agriculture. In light of the current situation, the aim of this study was to investigate the effects of thiamethoxam on the development of the reproductive system and physiology in the honey bee queen. Two experimental groups of honey bee queen larvae were treated with thiamethoxam during artificial rearing, applied via artificial feed in two cycles. In the first rearing cycle, honey bee larvae received a single treatment dose (4.28 ng thiamethoxam/queen larva on the 4th day after larvae grafting in artificial queen cells), while the second honey bee queen rearing cycle received a double treatment dose (total of 8.56 ng thiamethoxam/queen larva on the 4th and 5th day after larvae grafting in artificial queen cells). After emerging, queens were anesthetized and weighed, and after mating with drones were anesthetized, weighed, and sectioned. Ovary mass and number of stored sperm were determined. Body weight differed between untreated and treated honey bee queens. The results also show a decrease in the number of sperm within honey bee queen spermathecae that received the double thiamethoxam dose.
世界各地蜜蜂大量损失，部分原因与农业中频繁使用新烟碱类农药有关。鉴于当前形势，本研究旨在调查噻虫嗪对蜂王生殖系统发育和生理机能的影响。在人工饲养期间，通过人工饲料分两个周期对两组蜂王幼虫进行噻虫嗪处理。在第一个饲养周期，蜜蜂幼虫接受单次处理剂量（在人工王台中移入幼虫后第4天，每只蜂王幼虫给予4.28纳克噻虫嗪），而在第二个蜂王饲养周期，接受双倍处理剂量（在人工王台中移入幼虫后第4天和第5天，每只蜂王幼虫共给予8.56纳克噻虫嗪）。羽化后，对蜂王进行麻醉和称重，与雄蜂交尾后，再次对其进行麻醉、称重并解剖。测定卵巢质量和储存精子数量。未处理和经处理的蜂王体重存在差异。结果还显示，接受双倍噻虫嗪剂量的蜂王受精囊中精子数量减少。
