Tlak Gajger Ivana, Nejedli Srebrenka, Cvetnić Luka
Department for Biology and Pathology of Fish and Bees, Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, Croatia.
Department for Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, Croatia.
Microorganisms. 2023 Feb 28;11(3):610. doi: 10.3390/microorganisms11030610.
Honeybees' gut microbiota can provide new valuable access into the pathogenesis-related factors included in infections. Hence, we researched the presence and comparison of gut microbiota groups in control and spp.-infected honeybee colonies through high-throughput sequencing of the 16S rRNA. As the newest approach in apiary management, we hypothesize that the EM probiotic for bees could have an important role in therapeutic and immunomodulatory effects on honeybee colonies. The aim of this study was to estimate its impact on the gut microbiota composition of adult honeybees. The major genera were detected, where Lactobacillus was the most abundant genus, followed by Gilliamela, Snodgrassella, and Bifidobacterium. Inoculation with spp. spores made the relative proportions of Bifidobacterium lower, which was ameliorated by EM for bees' application. In addition, EM for bee applied treatments suppressed the increase in the number of spp. spores. This result points out that continuous EM for bees treatment shall change bees' gut microbiome composition and mitigate the influence of spp. infection. was a major member of the honeybee gut microbiota and may be significantly increased by long-term treatment with EM for bees. Toward these results, it is possible that EM for bees treatment will protect honeybees from herbicide glyphosate negative effects in agricultural fields by improving microbiome and immune functions.
蜜蜂的肠道微生物群可以为了解感染中涉及的发病相关因素提供新的有价值的途径。因此，我们通过对16S rRNA进行高通量测序，研究了对照蜂群和感染了[具体菌种]的蜂群中肠道微生物群的存在情况并进行了比较。作为养蜂管理中的最新方法，我们假设蜜蜂用EM益生菌可能对蜂群具有重要的治疗和免疫调节作用。本研究的目的是评估其对成年蜜蜂肠道微生物群组成的影响。检测到了主要的属，其中乳酸杆菌是最丰富的属，其次是吉氏菌属、斯诺德格拉斯菌属和双歧杆菌属。接种[具体菌种]孢子使双歧杆菌的相对比例降低，而蜜蜂用EM的应用改善了这种情况。此外，蜜蜂用EM处理抑制了[具体菌种]孢子数量的增加。这一结果表明，持续的蜜蜂用EM处理会改变蜜蜂的肠道微生物组组成，并减轻[具体菌种]感染的影响。[具体菌种]是蜜蜂肠道微生物群的主要成员，长期使用蜜蜂用EM处理可能会使其显著增加。基于这些结果，蜜蜂用EM处理有可能通过改善微生物组和免疫功能来保护蜜蜂免受农田中除草剂草甘膦的负面影响。
