College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, People's Republic of China.
Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193, Beijing, People's Republic of China.
Biol Trace Elem Res. 2018 Apr;182(2):354-363. doi: 10.1007/s12011-017-1097-9. Epub 2017 Jul 26.
Lead (Pb) is a toxic heavy metal and can harm organisms by inducing apoptosis. Selenium (Se), an essential trace element for humans and animals, can alleviate heavy metal toxicity. The aim of our study is to investigate alleviative effect of Se on Pb-induced apoptosis via endoplasmic reticulum (ER) stress in chicken kidneys. One hundred and eighty male chickens were randomly divided into four groups at 7 days of age and were fed with commercial diet (containing 0.49 mg/kg Se) and drinking water, NaSeO-added commercial diet (containing 1 mg/kg Se) and drinking water, the commercial diet and (CHOO)Pb-added drinking water (containing 350 mg/L Pb), and NaSeO-added commercial diet (containing 1 mg/kg Se) and (CHOO)Pb-added drinking water (containing 350 mg/L Pb), respectively. On the 30th, 60th, and 90th days of the experiment period, 15 chickens in each group were euthanized and the kidneys were collected. Following contents were performed: kidney ultrastructure; nitric oxide (NO) content; inducible nitric oxide synthase (iNOS) activity; relative messenger RNA (mRNA) and protein expression of iNOS, ER-related genes (glucose-regulated protein (GRP)78, GRP94, activating transcription factor (ATF)4, ATF6, and iron-responsive element (IRE)), and apoptosis-related genes (caspase-3 and B cell lymphoma-2 (Bcl-2)); and caspase-12 protein expression. The results indicated that Pb changed kidney ultrastructural structure; decreased Bcl-2 mRNA and protein expression; and increased NO content, iNOS activity, relative mRNA and protein expression of iNOS, ER-related genes, and caspase-3 and caspase-12 protein expression. Se attenuated above changes caused by Pb. Pb had time-dependent manners on NO content, GRP78, GRP94, ATF4, IRE, and caspase-3 mRNA expression. Se attenuated Pb-induced apoptosis via ER stress in the chicken kidneys.
铅（Pb）是一种有毒重金属，可通过诱导细胞凋亡对生物体造成伤害。硒（Se）是人和动物必需的微量元素，可减轻重金属毒性。本研究旨在探讨硒通过内质网（ER）应激减轻鸡肾脏中 Pb 诱导的细胞凋亡的缓解作用。7 日龄时，将 180 只雄性鸡随机分为 4 组，分别饲喂含 0.49mg/kg Se 的商业饲料和饮用水、含 1mg/kg Se 的 NaSeO 添加商业饲料和饮用水、含 350mg/L Pb 的商业饲料和（CHOO）Pb 添加饮用水，以及含 1mg/kg Se 的 NaSeO 添加商业饲料和（CHOO）Pb 添加饮用水。实验期第 30、60 和 90 天，每组 15 只鸡处死，采集肾脏。进行以下内容：肾脏超微结构；一氧化氮（NO）含量；诱导型一氧化氮合酶（iNOS）活性；iNOS、ER 相关基因（葡萄糖调节蛋白（GRP）78、GRP94、激活转录因子（ATF）4、ATF6 和铁反应元件（IRE））和凋亡相关基因（半胱天冬酶-3 和 B 细胞淋巴瘤-2（Bcl-2））的相对信使 RNA（mRNA）和蛋白表达；和半胱天冬酶-12 蛋白表达。结果表明，Pb 改变了肾脏超微结构；降低了 Bcl-2 mRNA 和蛋白表达；增加了 NO 含量、iNOS 活性、iNOS、ER 相关基因、半胱天冬酶-3 和半胱天冬酶-12 蛋白表达的相对 mRNA 和蛋白表达。Se 减轻了 Pb 引起的上述变化。Pb 对 NO 含量、GRP78、GRP94、ATF4、IRE 和 caspase-3 mRNA 表达具有时间依赖性。Se 通过 ER 应激减轻了鸡肾脏中 Pb 诱导的细胞凋亡。
