School of Biological Sciences, University of Tasmania, Sandy Bay, 7001, Australia.
Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, D-06466, Germany.
New Phytol. 2017 Oct;216(1):193-204. doi: 10.1111/nph.14690. Epub 2017 Jul 27.
In recent years the biosynthesis of auxin has been clarified with the aid of mutations in auxin biosynthesis genes. However, we know little about the effects of these mutations on the seed-filling stage of seed development. Here we investigate a key auxin biosynthesis mutation of the garden pea, which results in auxin deficiency in developing seeds. We exploit the large seed size of this model species, which facilitates the measurement of compounds in individual seeds. The mutation results in small seeds with reduced starch content and a wrinkled phenotype at the dry stage. The phenotypic effects of the mutation were fully reversed by introduction of the wild-type gene as a transgene, and partially reversed by auxin application. The results indicate that auxin is required for normal seed size and starch accumulation in pea, an important grain legume crop.
近年来，借助于生长素生物合成基因的突变，生长素的生物合成已经得到阐明。然而，我们对这些突变对种子发育的灌浆阶段的影响知之甚少。在这里，我们研究了豌豆中一个关键的生长素生物合成突变，该突变导致发育中的种子生长素缺乏。我们利用这个模式物种的大种子大小，这便于测量单个种子中的化合物。该突变导致种子变小，淀粉含量降低，在干燥阶段出现皱缩表型。突变的表型效应可以通过引入野生型基因作为转基因完全逆转，部分逆转可以通过施加生长素实现。结果表明，生长素是豌豆正常种子大小和淀粉积累所必需的，豌豆是一种重要的粮食豆类作物。
