Lindh B, Hökfelt T, Elfvin L G, Terenius L, Fahrenkrug J, Elde R, Goldstein M
J Neurosci. 1986 Aug;6(8):2371-83. doi: 10.1523/JNEUROSCI.06-08-02371.1986.
The topography of the peptidergic neuronal subpopulations in the guinea pig celiac-superior mesenteric ganglion was studied analyzing the distribution of immunoreactivity to neuropeptide Y (NPY), somatostatin (SOM), and vasoactive intestinal polypeptide (VIP)/polypeptide HI (PHI). For comparison, the ganglion was also studied using antisera against the 2 catecholamine-synthesizing enzymes tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (DBH). Approximately 65% of the neuronal cell bodies contained NPY-like immunoreactivity (NPY-LI), whereas 25% of the principal ganglion cells contained SOM-like immunoreactivity (SOM-LI). Though occasional cells were found to contain both NPY-LI and SOM-LI, these peptides had a complementary distribution in the ganglion, with NPY cells in the celiac poles and SOM cells in the superior mesenteric pole. The vast majority of both the NPY- and SOM-positive cells also contained TH-like immunoreactivity (TH-LI), confirming their catecholaminergic, presumably noradrenergic, nature. Some noradrenergic neurons seemed to lack NPY- and SOM-LI. Small numbers of VIP/PHI-containing cell bodies were found in areas where the NPY-immunoreactive neurons predominated. Many of the VIP/PHI-positive cells contained NPY-LI and occasionally also TH-LI. The immunohistochemical markers were also observed in fibers. Thus, a comparatively weak NPY-LI was seen in smooth fibers, probably representing axons and axon bundles. SOM-LI was seen in a similar type of fiber but also in more strongly fluorescent fibers with a varicose appearance. The latter fibers were observed only in the SOM-dominated part of the ganglion, often surrounding the ganglion cells. Varicose fibers with a similar distribution containing DBH-like immunoreactivity (DBH-LI) were also seen. In addition, DBH- and TH-LI were seen in smooth axonlike processes. VIP-positive fibers exhibited a very dense fiber network, almost exclusively related to the SOM cell-dominated part of the ganglion. The projection of the postganglionic sympathetic neurons was studied with special reference to the pylorus using a combination of retrograde axonal tracing and indirect immunofluorescence techniques. Seventy-two hours after injection of the fluorescent tracer Fast Blue into the pyloric sphincter, labeled neurons were found in the ganglion. By comparing the Fast Blue-labeled cells with the immunoreactive cell bodies, neurons containing both dye and NPY- or SOM-LI were observed. In elution-restaining experiments, it was established that the majority of these cells were also immunoreactive to TH, indicating that they produce noradrenaline.(ABSTRACT TRUNCATED AT 400 WORDS)
通过分析豚鼠腹腔-肠系膜上神经节中神经肽Y（NPY）、生长抑素（SOM）和血管活性肠肽（VIP）/肽组氨酸异亮氨酸（PHI）的免疫反应分布，研究了肽能神经元亚群的拓扑结构。为作比较，还使用抗两种儿茶酚胺合成酶酪氨酸羟化酶（TH）和多巴胺β-羟化酶（DBH）的抗血清对该神经节进行了研究。约65%的神经元细胞体含有NPY样免疫反应性（NPY-LI），而25%的主要神经节细胞含有SOM样免疫反应性（SOM-LI）。虽然偶尔发现细胞同时含有NPY-LI和SOM-LI，但这些肽在神经节中呈互补分布，腹腔极有NPY细胞，肠系膜上极有SOM细胞。绝大多数NPY和SOM阳性细胞也含有TH样免疫反应性（TH-LI），证实了它们的儿茶酚胺能性质，推测为去甲肾上腺素能性质。一些去甲肾上腺素能神经元似乎缺乏NPY和SOM-LI。在NPY免疫反应性神经元占主导的区域发现了少量含VIP/PHI的细胞体。许多VIP/PHI阳性细胞含有NPY-LI，偶尔也含有TH-LI。在纤维中也观察到了免疫组化标记物。因此，在平滑肌纤维中可见相对较弱的NPY-LI，可能代表轴突和轴突束。在类似类型的纤维中可见SOM-LI，但也见于外观呈曲张状、荧光更强的纤维中。后一种纤维仅在神经节中以SOM为主的部分观察到，常围绕神经节细胞。还可见到分布类似、含有DBH样免疫反应性（DBH-LI）的曲张纤维。此外，在光滑的轴突样突起中可见DBH和TH-LI。VIP阳性纤维呈现出非常密集的纤维网络，几乎完全与神经节中以SOM细胞为主的部分相关。使用逆行轴突追踪和间接免疫荧光技术相结合的方法，特别参照幽门研究了节后交感神经元的投射。将荧光示踪剂快蓝注入幽门括约肌72小时后，在神经节中发现了标记神经元。通过将快蓝标记的细胞与免疫反应性细胞体进行比较，观察到了同时含有染料和NPY或SOM-LI的神经元。在洗脱保留实验中确定，这些细胞中的大多数对TH也有免疫反应，表明它们产生去甲肾上腺素。（摘要截短至400字）
