Morris J L, Gibbins I L, Furness J B
Department of Anatomy & Histology, Flinders University of South Australia, Bedford Park.
J Auton Nerv Syst. 1987 Nov;21(1):15-27. doi: 10.1016/0165-1838(87)90088-9.
We have reinvestigated the immunohistochemistry of autonomic axons supplying the guinea-pig uterine artery to determine whether non-noradrenergic paracervical ganglion neurons projecting to the artery contain immunoreactivity to dopamine-beta-hydroxylase (DBH) or somatostatin (SOM) in addition to neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP). In untreated arteries no VIP axons had immunoreactivity to tyrosine hydroxylase (TH), although 9% had immunoreactivity to DBH. Somatostatin immunoreactivity was detected in 25% of non-noradrenergic axons containing NPY and VIP. After in vivo treatment with 6-hydroxydopamine (6-OHDA), noradrenergic axons containing immunoreactivity to NPY, DBH and TH were absent from the adventitia-medial junction. However, 65-70% of the non-noradrenergic axons with NPY and VIP showed DBH immunoreactivity after 6-OHDA. These axons did not show catecholamine fluorescence after incubation with pargyline together with noradrenaline, dopamine or L-DOPA. The number of axons with SOM immunoreactivity increased by 44% after 6-OHDA treatment, but only 24% of SOM axons had DBH immunoreactivity. Surgical destruction of the non-noradrenergic autonomic axons in 6-OHDA-treated animals led to the loss of all DBH immunoreactivity. These results demonstrate that DBH immunoreactivity can be detected in a small proportion of non-noradrenergic axons supplying uterine arteries from untreated animals. After chemical sympathectomy with 6-OHDA, the levels of DBH immunoreactivity in axons of non-noradrenergic neurons increased, and more axons with DBH immunoreactivity were detected. DBH immunoreactivity seemed to increase preferentially in axons with NPY and VIP, but not SOM. The number of NPY, VIP axons containing SOM also increased after 6-OHDA. These findings demonstrate that peripheral neurons containing several different potential neurotransmitters can change their levels of neuropeptides and transmitter-synthesizing enzymes in response to local environmental changes.
我们重新研究了支配豚鼠子宫动脉的自主神经轴突的免疫组织化学，以确定投射至该动脉的非去甲肾上腺素能子宫颈旁神经节神经元除了含有神经肽Y（NPY）和血管活性肠肽（VIP）外，是否还含有多巴胺-β-羟化酶（DBH）或生长抑素（SOM）的免疫反应性。在未经处理的动脉中，没有VIP轴突对酪氨酸羟化酶（TH）有免疫反应性，尽管9%的VIP轴突对DBH有免疫反应性。在含有NPY和VIP的非去甲肾上腺素能轴突中，25%检测到生长抑素免疫反应性。在用6-羟基多巴胺（6-OHDA）进行体内处理后，在外膜-中膜交界处没有发现含有对NPY、DBH和TH有免疫反应性的去甲肾上腺素能轴突。然而，在用6-OHDA处理后，65%-70%含有NPY和VIP的非去甲肾上腺素能轴突显示出DBH免疫反应性。在用帕吉林与去甲肾上腺素、多巴胺或左旋多巴一起孵育后，这些轴突没有显示出儿茶酚胺荧光。在用6-OHDA处理后，含有SOM免疫反应性的轴突数量增加了44%，但只有24%的SOM轴突有DBH免疫反应性。在6-OHDA处理的动物中，对非去甲肾上腺素能自主神经轴突进行手术破坏导致所有DBH免疫反应性丧失。这些结果表明，在未经处理的动物供应子宫动脉的一小部分非去甲肾上腺素能轴突中可以检测到DBH免疫反应性。在用6-OHDA进行化学交感神经切除后，非去甲肾上腺素能神经元轴突中的DBH免疫反应性水平增加，并且检测到更多具有DBH免疫反应性的轴突。DBH免疫反应性似乎在含有NPY和VIP的轴突中优先增加，而在含有SOM的轴突中则不然。在用6-OHDA处理后，含有SOM的NPY、VIP轴突数量也增加了。这些发现表明，含有几种不同潜在神经递质的外周神经元可以响应局部环境变化而改变其神经肽和递质合成酶的水平。
