内毒素的电动特性及其在鲎试剂检测中的意义

Electrokinetic properties of endotoxins and their significance for the limulus amoebocyte lysate test.

作者信息

Baggerman C, Bonekamp B C, Kannegieter E M, Loos J A, Junginger H E

出版信息

J Pharm Pharmacol. 1986 Jul;38(7):510-4. doi: 10.1111/j.2042-7158.1986.tb04625.x.

DOI:10.1111/j.2042-7158.1986.tb04625.x

Abstract

The electrokinetic properties of the endotoxins of Escherichia coli and Serratia marcescens have been examined. Both endotoxins are negatively charged, with the zetapotential being increased by the presence of cations whose relative influence resembles the Schulze-Hardy rule for colloid stability. Fe3+ and Th4+ ions are capable of reversing the negative charge of the endotoxin particles to positive. These cations were found to have a strong inhibitory effect on the activity of endotoxins in the limulus amoebocyte lysate test but the inhibitory effect did not parallel changes in the zetapotential because the effect occurred at concentrations too low to affect this parameter.

摘要

对大肠杆菌和粘质沙雷氏菌内毒素的电动特性进行了研究。两种内毒素均带负电荷，阳离子的存在会增加其ζ电位，其相对影响类似于胶体稳定性的舒尔茨 - 哈迪规则。Fe3+和Th4+离子能够将内毒素颗粒的负电荷反转成正电荷。发现这些阳离子在鲎试剂试验中对内毒素的活性有很强的抑制作用，但抑制作用与ζ电位的变化不平行，因为这种作用发生在浓度过低而无法影响该参数的情况下。