组胺过敏反应与鲎试剂检测内毒素活性的比较。
Comparison of the histamine hypersensitivity and the Limulus amoebocyte lysate tests for endotoxin activity.
作者信息
Munoz J J, Bergman R K, Robbins K E
出版信息
Infect Immun. 1978 Oct;22(1):292-4. doi: 10.1128/iai.22.1.292-294.1978.
Abstract
The histamine hypersensitivity test and the Limulus amoebocyte lysate test were compared for their effectiveness to quantitate endotoxin activity. The two tests compared favorably in all the trials, except with a sample of endotoxin from Brucella abortus that gave a positive Limulus amoebocyte lysate test at a concentration of 0.001 microgram, while failing to sensitize mice to histamine at a dose of 16 microgram per mouse. The Limulus amoebocyte lysate test was more sensitive than the histamine hypersensitivity test.
摘要
对组胺过敏试验和鲎试剂试验在定量内毒素活性方面的有效性进行了比较。除了一份流产布鲁氏菌内毒素样本外，在所有试验中这两种试验的结果都比较理想。该样本在浓度为0.001微克时鲎试剂试验呈阳性，但以每只小鼠16微克的剂量却未能使小鼠对组胺敏感。鲎试剂试验比组胺过敏试验更敏感。
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