Marine Biophysics Unit, Okinawa Institute of Science and Technology Graduate University, Tancha 1919-1, Onna, Okinawa, 904-0495, Japan.
Okinawa Marine Science Support Section, Okinawa Institute of Science and Technology Graduate University, Tancha 1919-1, Onna, Okinawa, 904-0495, Japan.
Sci Rep. 2017 Jul 27;7(1):6729. doi: 10.1038/s41598-017-06776-x.
Population genetics of the coral genus Pocillopora have been more intensively studied than those of any other reef-building taxon. However, recent investigations have revealed that the current morphological classification is inadequate to represent genetic lineages. In this study, we isolated and characterized novel microsatellite loci from morphological Pocillopora meandrina (Type 1) and Pocillopora acuta (Type 5). Furthermore, we characterized previously reported microsatellite loci. A total of 27 loci (13 novel loci) proved useful for population genetic analyses at two sites in the Ryukyu Archipelago, in the northwestern Pacific. Clonal diversity differed in each genetic lineage. Genetic structure suggested by microsatellites corresponded to clusters in a phylogenetic tree constructed from a mitochondrial open reading frame (mtORF). In addition, we found an unknown mitochondrial haplotype of this mtORF. These microsatellite loci will be useful for studies of connectivity and genetic diversity of Pocillopora populations, and will also support coral reef conservation.
与其他任何造礁生物相比，人们对珊瑚属的群体遗传学进行了更为深入的研究。然而，最近的调查显示，当前的形态分类不足以代表遗传谱系。在这项研究中，我们从形态学上的 Pocillopora meandrina（Type 1）和 Pocillopora acuta（Type 5）中分离和鉴定了新的微卫星基因座。此外，我们还对之前报道的微卫星基因座进行了鉴定。在西北太平洋的琉球群岛的两个地点的种群遗传分析中，27 个基因座（13 个新基因座）证明是有用的。各遗传谱系的无性系多样性存在差异。微卫星显示的遗传结构与从线粒体开放阅读框（mtORF）构建的系统发育树中的聚类相对应。此外，我们还发现了该 mtORF 的一个未知的线粒体单倍型。这些微卫星基因座将有助于研究 Pocillopora 种群的连通性和遗传多样性，并支持珊瑚礁保护。
