State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin 300353, P. R. China.
Chem Commun (Camb). 2017 Aug 10;53(65):9075-9077. doi: 10.1039/c7cc04362g.
A novel enzymatic approach was developed for facile production of glycopeptides carrying natural eukaryotic N-glycans. In this approach, peptides can be GlcNAcylated at one or two natural N-glycosylation sites via two-step enzymatic reactions catalyzed by an evolved N-glycosyltransferase (ApNGT) and a glucosamine N-acetyltransferase (GlmA), respectively. The resulting GlcNAc-peptides were further modified by an endo-β-N-acetylglucosaminidase M mutant (EndoM) to generate glycopeptides. In three steps of enzymatic catalysis, glycopeptides carrying complex-type N-glycans can be efficiently synthesized.
开发了一种新颖的酶法，可轻松制备带有天然真核 N-聚糖的糖肽。在此方法中，通过两步酶促反应，分别由经过改造的 N-糖基转移酶 (ApNGT) 和葡萄糖胺 N-乙酰基转移酶 (GlmA) 催化，可使肽在一个或两个天然 N-糖基化位点上发生 GlcNAc 化。所得的 GlcNAc-肽进一步通过内切-β-N-乙酰氨基葡萄糖苷酶 M 突变体 (EndoM) 修饰，以生成糖肽。通过三步酶促催化，可以有效地合成带有复杂型 N-聚糖的糖肽。
