阿米卡星对脓肿分枝杆菌细胞壁糖肽脂合成的影响。
Effect of amikacin on cell wall glycopeptidolipid synthesis in Mycobacterium abscessus.
作者信息
Lee So-Young, Kim Hee-Youn, Kim Byoung-Jun, Kim Hong, Seok Seung-Hyeok, Kim Bum-Joon, Kook Yoon-Hoh
机构信息
Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea.
Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea.
出版信息
J Microbiol. 2017 Aug;55(8):640-647. doi: 10.1007/s12275-017-6503-7. Epub 2017 Jul 28.
Cultivation of the smooth colony Mycobacterium abscessus at the sub-minimum inhibitory concentration (MIC) of amikacin changed its growth pattern including its colony morphology (smooth to rough) and cell arrangement (dispersed to cord formation). In addition, reduced sliding motility and biofilm formation were observed. The amount of glycogpetidolipid (GPL) and mRNA expression of key genes involved in GPL synthesis were decreased in the amikacin-treated M. abscessus strain. An in vitro infection assay revealed that the amikacin-treated smooth M. abscessus strain induced more pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) than that of the smooth strain in murine macrophage cells. These results suggest that long-term exposure to a low concentration of amikacin causes a physical change in the cell wall which may increase its virulence.
在低于阿米卡星最低抑菌浓度（MIC）的条件下培养光滑菌落型脓肿分枝杆菌，会改变其生长模式，包括菌落形态（从光滑变为粗糙）和细胞排列（从分散变为索状形成）。此外，还观察到滑动运动性和生物膜形成减少。在经阿米卡星处理的脓肿分枝杆菌菌株中，糖肽脂（GPL）的量以及参与GPL合成的关键基因的mRNA表达均降低。体外感染试验表明，经阿米卡星处理的光滑型脓肿分枝杆菌菌株在鼠巨噬细胞中诱导产生的促炎细胞因子（TNF-α、IL-1β、IL-6）比光滑菌株更多。这些结果表明，长期暴露于低浓度的阿米卡星会导致细胞壁发生物理变化，这可能会增加其毒力。
Zotero 插件