Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
Tongji University School of Medicine, Shanghai, China.
J Infect Dis. 2020 Mar 16;221(Suppl 2):S257-S262. doi: 10.1093/infdis/jiz475.
Smooth and rough colony morphotypes of Mycobacterium abscessus are associated with virulence, but some isolates form both smooth and rough colonies, impeding successful morphotype identification. Reportedly, smooth/rough morphotypes are also related to the glycopeptidolipid (GPL) genotype. However, the accuracy of GPL genotyping to discriminate morphotypes and the relationship between GPL genotype and clinical characteristics of M abscessus lung disease have not been verified.
A retrospective analysis of colony morphology, GPL genotype, and clinical data from 182 patients with M abscessus lung disease was conducted.
Of 194 clinical isolates, 126 (65.0%), 15 (7.7%), and 53 (27.3%) exhibited rough, smooth, and mixed colony morphotypes, respectively. Glycopeptidolipid genotyping indicated that 86.7% (13 of 15) of smooth isolates belonged to the GPL-wild type (WT) group, whereas 98.4% (124 of 126) of rough isolates belonged to the GPL-mutant type (MUT) group. Therefore, GPL genotyping accurately distinguished between smooth and rough morphotypes. Mixed colony morphotypes were also divided into GPL-WT (18.9%) and GPL-MUT (81.1%) groups. Further analysis revealed that patients infected with the GPL-MUT group presented with significantly worse baseline clinical characteristics and exacerbated episodes of lung disease.
Glycopeptidolipid genotyping accurately distinguishes smooth and rough colony morphotypes. Patients infected with the GPL-MUT genotype exhibit worse clinical characteristics and are at a higher risk of exacerbated lung disease.
脓肿分枝杆菌的光滑和粗糙菌落形态与毒力相关，但有些分离株同时形成光滑和粗糙菌落，这阻碍了对形态的成功鉴定。据报道，光滑/粗糙形态也与糖肽脂（GPL）基因型有关。然而，GPL 基因分型对区分形态的准确性以及 GPL 基因型与脓肿分枝杆菌肺病的临床特征之间的关系尚未得到验证。
对 182 例脓肿分枝杆菌肺病患者的菌落形态、GPL 基因型和临床数据进行回顾性分析。
在 194 株临床分离株中，分别有 126 株（65.0%）、15 株（7.7%）和 53 株（27.3%）表现为粗糙、光滑和混合菌落形态。糖肽脂基因分型表明，15 株光滑分离株中有 13 株（86.7%）属于 GPL 野生型（WT）组，而 126 株粗糙分离株中有 124 株（98.4%）属于 GPL 突变型（MUT）组。因此，GPL 基因分型能准确区分光滑和粗糙形态。混合菌落形态也分为 GPL-WT（18.9%）和 GPL-MUT（81.1%）组。进一步分析显示，感染 GPL-MUT 组的患者基线临床特征更差，肺病恶化发作更频繁。
糖肽脂基因分型能准确区分光滑和粗糙菌落形态。感染 GPL-MUT 基因型的患者临床特征更差，肺病恶化的风险更高。
