Yang Yu-Bo, Bai Yun-Jin, Tang Yin, Liu Zhen-Hua, Wei Qiang, Han Ping
Department of Urology, Insititute of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2017 Jul;48(4):605-609.
To detect circulating tumor cells (CTCs) in peripheral blood from the patients with bladder cancer using subtraction enrichment and immunostaining-fluorescence in situ hybridization (SE-iFISH) and to explore the relation between CTCs detection result and the clinicpathological parameters of tumors.
Sixteen patients [(66.88±8.97) yr.] with bladder cancerwere included, whlile 10 normal people [(61.90±9.13) yr.] were enrolled as control. Peripheral blood was harvested (6 mL per case) for the detection of CTCs with the method of subtraction enrichment and SE-iFISH, and the correlations of CTCs level to the clinicpathological parameters of tumors were analyzed, including hematuria, size, number, NMIBC or MIBC, stage, grade and pathological type.
Positive rates of CTCs in experimental and control group were 43.75% (7/16) and 0% (0/10), respectively. The difference was statistically significant (=0.022 7). The number of CTCS was not associated with the clinicpathological parameters of bladder cancer, which probably due to small sample size.
It is applicable to detect CTCs of bladder cancer by SE-iFISH.
采用消减富集和免疫染色-荧光原位杂交（SE-iFISH）技术检测膀胱癌患者外周血中的循环肿瘤细胞（CTC），并探讨CTC检测结果与肿瘤临床病理参数之间的关系。
纳入16例膀胱癌患者[年龄（66.88±8.97）岁]，同时选取10例正常人[年龄（61.90±9.13）岁]作为对照。采集外周血（每例6 mL），采用消减富集和SE-iFISH方法检测CTC，并分析CTC水平与肿瘤临床病理参数（包括血尿、大小、数量、非肌层浸润性膀胱癌或肌层浸润性膀胱癌、分期、分级和病理类型）的相关性。
实验组和对照组CTC的阳性率分别为43.75%（7/16）和0%（0/10）。差异具有统计学意义（P=0.022 7）。CTC数量与膀胱癌的临床病理参数无关，这可能是由于样本量较小。
SE-iFISH技术适用于检测膀胱癌的CTC。
