Sheng Yuan, Wang Ting, Li Hengyu, Zhang Zhenzhen, Chen Jianghao, He Chenyang, Li Yongping, Lv Yonggang, Zhang Juliang, Xu Cheng, Wang Zhen, Huang Chen, Wang Ling
Department of Thyroid and Breast Surgery, Changhai Hospital, The Second Military Medical University, Shanghai, China.
Department of Vascular and Endocrine Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China.
Oncotarget. 2017 Jan 31;8(5):8801-8806. doi: 10.18632/oncotarget.6688.
Circulating tumor cells (CTCs) have been widely used to predict the prognosis of breast cancer patients. The aim of the present study was to compare the performances of Cellsearch and immunostaining-fluorescence in situ hybridization (iFISH) in detecting CTCs in breast cancer patients. Forty-five newly diagnosed breast cancer patients and 14 healthy donors were recruited and their CTCs were detected by both Cellsearch and iFISH. Correlation between clinicopathological features and CTCs was investigated. We found that the positive rate of CTC detected by iFISH was significantly higher than by Cellsearch system (91% vs 38%). The CTC count, detected either by iFISH or Cellsearch, was not significantly associated with clinical pictures of patients with breast cancer. Therefore, we concluded that, compared to conventional Cellsearch CTC detection, in situ karyotypic identification performed by iFISH had higher detection rate. Therefore, iFISH may be more clinically useful than Cellsearch system.
循环肿瘤细胞（CTCs）已被广泛用于预测乳腺癌患者的预后。本研究的目的是比较Cellsearch和免疫染色荧光原位杂交（iFISH）在检测乳腺癌患者循环肿瘤细胞方面的性能。招募了45例新诊断的乳腺癌患者和14名健康供体，并用Cellsearch和iFISH检测他们的循环肿瘤细胞。研究了临床病理特征与循环肿瘤细胞之间的相关性。我们发现，iFISH检测到的循环肿瘤细胞阳性率显著高于Cellsearch系统（91%对38%）。通过iFISH或Cellsearch检测到的循环肿瘤细胞计数与乳腺癌患者的临床表现无显著相关性。因此，我们得出结论，与传统的Cellsearch循环肿瘤细胞检测相比，iFISH进行的原位核型鉴定具有更高的检测率。因此，iFISH在临床上可能比Cellsearch系统更有用。
