高等植物线粒体中信使核糖核酸的转录本末端
Transcript termini of messenger RNAs in higher plant mitochondria.
作者信息
Schuster W, Hiesel R, Isaac P G, Leaver C J, Brennicke A
出版信息
Nucleic Acids Res. 1986 Aug 11;14(15):5943-54. doi: 10.1093/nar/14.15.5943.
The 3'-termini of the mRNAs for subunit II of the cytochrome oxidase (COX II) and for the alpha-subunit of the mitochondrial ATPase (ATPA) have been determined in Oenothera mitochondria by two independent methods. Analysis of both transcripts by S1 protection experiments and of cloned cDNAs show an identical terminal 50 nucleotide sequence, to which homology is found 3' to some gene sequences in the maize mitochondrial genome. These regions can be folded into potential secondary structures similar to bacterial terminators.
通过两种独立的方法，已确定了月见草属植物线粒体中细胞色素氧化酶亚基II（COX II）和线粒体ATP酶α亚基（ATPA）的mRNA的3'末端。通过S1保护实验对这两种转录本以及对克隆的cDNA进行分析，结果显示它们具有相同的50个核苷酸的末端序列，在玉米线粒体基因组中的某些基因序列3'端发现了与之同源的序列。这些区域可以折叠成类似于细菌终止子的潜在二级结构。
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