直接从阳性富集肉汤中进行基质辅助激光解吸电离飞行时间质谱鉴定及抗菌药敏试验。
MALDI-TOF MS identification and antimicrobial susceptibility testing directly from positive enrichment broth.
作者信息
Tré-Hardy Marie, Lambert Barbara, Despas Noémie, Bressant Florian, Laurenzano Clémentine, Rodriguez-Villalobos Hector, Verroken Alexia
机构信息
Department of Microbiology, Cliniques Universitaires Saint-Luc, Université Catholique de Louvain, Brussels, Belgium.
Department of Microbiology, Cliniques Universitaires Saint-Luc, Université Catholique de Louvain, Brussels, Belgium.
出版信息
J Microbiol Methods. 2017 Oct;141:32-34. doi: 10.1016/j.mimet.2017.07.012. Epub 2017 Jul 25.
A rapid preparation procedure was validated for MALDI-TOF MS identification followed by an antimicrobial susceptibility testing directly from a non-selective enrichment broth of sterile fluid samples associated with negative classical cultures. This method can be easily integrated in the laboratory routine allowing precious time gain for microbe identification and subsequent adequate treatment initiation.
一种快速制备程序经验证可用于基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定，随后直接从与经典培养阴性相关的无菌液体样本的非选择性富集肉汤中进行抗菌药敏试验。该方法可轻松整合到实验室常规操作中，从而为微生物鉴定和随后开始适当治疗节省宝贵时间。
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