Manandhar Pooja, Zhang Gengxin, Lama Arun, Liu Fei, Hu Yilun
Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
Antonie Van Leeuwenhoek. 2017 Dec;110(12):1659-1668. doi: 10.1007/s10482-017-0915-6. Epub 2017 Jul 28.
An orange pigmented, Gram-staining negative, aerobic, motile, rod-shaped bacterium isolated from a soil from the Tanggula Mountain, China was studied using a polyphasic approach. Based on 16S rRNA gene sequence similarity, strain W16RD was found to be closely related to Sphingomonas prati DSM 103336 (99%), Sphingomonas fennica DSM 13665 (97.21%), followed by Sphingomonas laterariae DSM 25432 (96.44%), Sphingomonas haloaromaticamans CGMCC 1.10206 (96.36%) and Sphingomonas formosensis DSM 24164 (96.06%). The strain was found to be catalase and oxidase positive and was found to grow optimally at temperatures of 20-25 °C, pH 8 and tolerated NaCl concentration up to 1% (w/v). The major fatty acids identified were summed feature eight comprising C ω 7c and/or C ω 6c (39.2%), summed feature three comprising of C ω7c and/or C ω6c (36.7%) and C (7.0%). The polar lipids detected were phosphatidylcholine, sphingoglycolipid, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidyldimethylethanolamine, phosphatidylmonomethylethanolamine, and three unidentified lipids. The strain possessed ubiquinone-10 (Q-10) as the predominant respiratory quinone. Along with other distinguishing characteristics, we also describe the draft genome of strain W16RD. The final assembled draft genome sequence is 3,722,743 bp with 3390 coding and 48 RNA (45 tRNA and 3 rRNA) genes. The DNA G+C content of the genomic DNA was determined to be 67%. The DNA-DNA relatedness value between the strain W16RD and its closest phylogenetic relatives S. prati DSM 103336, S. fennica DSM 13665, S. laterariae DSM 25432, and S. haloaromaticamans CGMCC 1.10206 were 52.17, 47.60, 20.93 and 17.09% respectively. The strain W16RD could be distinguished genotypically and phenotypically from the recognized species belonging to the genus Sphingomonas and thus represents a novel species, for which the name Sphingomonas montana sp. nov. is proposed. The type strain is W16RD (=CGMCC 1.15646 = DSM 103337).
从中国唐古拉山的土壤中分离出一株橙色色素沉着、革兰氏染色阴性、需氧、运动、杆状的细菌，并采用多相方法对其进行了研究。基于16S rRNA基因序列相似性，发现菌株W16RD与草地鞘氨醇单胞菌DSM 103336（99%）、芬兰鞘氨醇单胞菌DSM 13665（97.21%）密切相关，其次是侧生鞘氨醇单胞菌DSM 25432（96.44%）、卤代芳烃鞘氨醇单胞菌CGMCC 1.10206（96.36%）和台湾鞘氨醇单胞菌DSM 24164（96.06%）。该菌株过氧化氢酶和氧化酶呈阳性，在20 - 25℃、pH 8的条件下生长最佳，耐受高达1%（w/v）的NaCl浓度。鉴定出的主要脂肪酸为总和特征八（包含C ω 7c和/或C ω 6c，占39.2%）、总和特征三（包含C ω7c和/或C ω6c，占36.7%）以及C18:1ω7c（占7.0%）。检测到的极性脂质有磷脂酰胆碱、鞘糖脂、磷脂酰甘油、磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰二甲基乙醇胺、磷脂酰单甲基乙醇胺以及三种未鉴定的脂质。该菌株以泛醌-10（Q-10）作为主要呼吸醌。连同其他鉴别特征，我们还描述了菌株W16RD的基因组草图。最终组装的基因组草图序列为3,722,743 bp，有3390个编码基因和48个RNA（45个tRNA和3个rRNA）基因。基因组DNA的G+C含量测定为67%。菌株W16RD与其最接近的系统发育亲缘种草地鞘氨醇单胞菌DSM 103336、芬兰鞘氨醇单胞菌DSM 13665、侧生鞘氨醇单胞菌DSM 25432和卤代芳烃鞘氨醇单胞菌CGMCC 1.10206之间的DNA-DNA相关性值分别为52.17%、47.60%、20.93%和17.09%。菌株W16RD在基因型和表型上可与鞘氨醇单胞菌属中已认可的物种区分开来，因此代表一个新物种，为此提出名为蒙氏鞘氨醇单胞菌（Sphingomonas montana sp. nov.）。模式菌株为W16RD（=CGMCC 1.15646 = DSM 103337）。
