Chaudhary Dhiraj Kumar, Dahal Ram Hari, Kim Jaisoo
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do, 16227, South Korea.
Curr Microbiol. 2018 Oct;75(10):1299-1305. doi: 10.1007/s00284-018-1524-y. Epub 2018 Jun 5.
Strain DRJ-4 was isolated from forest soil of a low-altitude mountain and was taxonomically characterized by a polyphasic approach. This strain was yellow-colored, Gram-stain-negative, strictly aerobic, motile, non-sporulating, catalase-positive, oxidase-negative, and rod-shaped. Strain DRJ-4 was able to grow at 20-42 °C, pH 6.5-10.0, and 0-1.0% (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain DRJ-4 formed a distinct lineage within the members of the family Sphingomonadaceae of the phylum Proteobacteria and moderately related to Sphingomonas faucium E62-3 (96.67% sequence similarity), 'Sphingosinicella ginsenosidimutans' BS11 (96.45% sequence similarity), Sphingosinicella vermicomposti YC7378 (96.27% sequence similarity), and Sphingobium boeckii 469 (95.82% sequence similarity). The sole respiratory quinone was ubiquinone Q-10 and the major polyamine was homospermidine. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, and sphingoglycolipid. The predominant fatty acids of strain DRJ-4 were summed feature 8 (Cω7c and/or Cω6c), summed feature 3 (Cω7c and/or Cω6c), C, and Cω6c. The genomic DNA G + C was 64.0 mol %. On the basis of phenotypic, genotypic, and phylogenetic analysis, strain DRJ-4 represents a novel species of the genus Sphingomonas, for which the name Sphingomonas montis sp. nov. is proposed. The type strain of Sphingomonas montis is DRJ-4 (= KEMB 9005-708 = NBRC 113142).
菌株DRJ-4从低海拔山区的森林土壤中分离得到，并采用多相分类法进行分类鉴定。该菌株呈黄色，革兰氏染色阴性，严格需氧，具运动性，不产芽孢，过氧化氢酶阳性，氧化酶阴性，杆状。菌株DRJ-4能够在20-42℃、pH 6.5-10.0以及0-1.0%（w/v）NaCl浓度下生长。基于16S rRNA基因序列分析，菌株DRJ-4在变形菌门鞘脂单胞菌科成员中形成一个独特的分支，与福氏鞘脂单胞菌E62-3（序列相似性96.67%）、“人参皂苷转化鞘脂小单胞菌”BS11（序列相似性96.45%）、蚯蚓堆肥鞘脂小单胞菌YC7378（序列相似性96.27%）以及博氏鞘氨醇杆菌469（序列相似性95.82%）中度相关。唯一的呼吸醌为泛醌Q-10，主要的多胺为高亚精胺。极性脂质谱显示存在磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、磷脂酰胆碱、磷脂酰单甲基乙醇胺和鞘糖脂。菌株DRJ-4的主要脂肪酸为总和特征8（Cω7c和/或Cω6c）、总和特征3（Cω7c和/或Cω6c）、C和Cω6c。基因组DNA的G+C含量为64.0 mol%。基于表型、基因型和系统发育分析，菌株DRJ-4代表鞘脂单胞菌属的一个新物种，为此提出新名称鞘脂单胞菌山地种（Sphingomonas montis sp. nov.）。鞘脂单胞菌山地种的模式菌株为DRJ-4（=KEMB 9005-708=NBRC 113142）。
