用于膜蛋白核磁共振研究的甲基特异性同位素标记策略
Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins.
作者信息
Kurauskas Vilius, Schanda Paul, Sounier Remy
机构信息
IBS, Université Grenoble Alpes, F-38044, Grenoble, France.
CEA, Institut de Biologie Structurale, F-38044, Grenoble, France.
出版信息
Methods Mol Biol. 2017;1635:109-123. doi: 10.1007/978-1-4939-7151-0_6.
Abstract
Methyl groups are very useful probes of structure, dynamics, and interactions in protein NMR spectroscopy. In particular, methyl-directed experiments provide high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking environment. In this chapter, we discuss the approach for labeling methyl groups in E. coli-based protein expression, as exemplified with the mitochondrial carrier GGC.
摘要
甲基基团是蛋白质核磁共振光谱中用于研究结构、动力学和相互作用的非常有用的探针。特别是，甲基导向实验即使在非常大的蛋白质中也能提供高灵敏度，比如在模拟膜环境中的膜蛋白。在本章中，我们将以线粒体载体GGC为例，讨论在基于大肠杆菌的蛋白质表达中标记甲基基团的方法。
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