Motta Erick Vicente da Silva, da Costa Juliana de Carvalho, Bastos Jairo Kenupp
School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil.
School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Sep 1;1061-1062:240-247. doi: 10.1016/j.jchromb.2017.07.027. Epub 2017 Jul 18.
Copaifera langsdorffii Desf. (Fabaceae, Caesalpinioideae), popularly known as "copaiba" or "pau d'óleo", is a species of tree that is found throughout Brazil. The leaves of this tree are used in folk medicine to treat kidney stones. Galloylquinic acid derivatives and flavonoids are the main secondary metabolites found in C. langsdorffii leaves and are likely to be responsible for the effectiveness of this treatment. As an attempt to produce a phytotherapic, we have developed a reliable HPLC-UV method for the quality control of C. langsdorffii leaves. Phenolic compounds were extracted from C. langsdorffii leaves using 70% aqueous ethanol as the extraction solvent. HPLC-UV analyses were carried out on a Synergi Polar-RP column (100×3.0mm, 2.5μm), and the mobile phase was made up of formic acid-water (0.1:99.9, solvent A), and isopropanol-methanol-acetonitrile (5:40:60, solvent B). The elution gradient was A:B (90:10 to 85:15) in 8.0min, followed by A:B (85:15 to 64:36) up to 30.0min, using a flow rate of 0.7mL/min, and UV detection at 280nm. This method was used to quantify nine galloylquinic acid derivatives and two flavonoids, which gave a good detection response and linearity in the range of 1.88-110.0μg/mL. Furthermore, the detection and quantification limits ranged from 0.070 to 0.752μg/mL, and 0.211-2.278μg/mL respectively, with a maximum RSD of 4.18%. The method is reliable for the quality control of C. langsdorffii raw material, its hydroethanolic extract, and could potentially be used to quantify these compounds in other Copaifera species.
朗氏南美苏铁（豆科，云实亚科），俗称“苦配巴”或“油树”，是一种遍布巴西的树种。这种树的叶子在民间医学中用于治疗肾结石。没食子酰奎宁酸衍生物和黄酮类化合物是在朗氏南美苏铁叶子中发现的主要次生代谢产物，可能是这种治疗方法有效性的原因。作为生产一种植物疗法的尝试，我们开发了一种可靠的高效液相色谱 - 紫外检测法用于朗氏南美苏铁叶子的质量控制。使用70%乙醇水溶液作为提取溶剂从朗氏南美苏铁叶子中提取酚类化合物。高效液相色谱 - 紫外检测分析在Synergi Polar - RP柱（100×3.0mm，2.5μm）上进行，流动相由甲酸 - 水（0.1:99.9，溶剂A）和异丙醇 - 甲醇 - 乙腈（5:40:60，溶剂B）组成。洗脱梯度为8.0分钟内A:B（90:10至85:15），然后至30.0分钟时A:B（85:15至64:36），流速为0.7mL/min，紫外检测波长为280nm。该方法用于定量九种没食子酰奎宁酸衍生物和两种黄酮类化合物，在1.88 - 110.0μg/mL范围内给出了良好的检测响应和线性关系。此外，检测限和定量限分别为0.070至0.752μg/mL和0.211 - 2.278μg/mL，最大相对标准偏差为4.18%。该方法对于朗氏南美苏铁原材料及其水乙醇提取物的质量控制是可靠的，并且有可能用于定量其他南美苏铁属物种中的这些化合物。
