School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
Phytochem Anal. 2019 May;30(3):364-372. doi: 10.1002/pca.2819. Epub 2019 Jan 15.
Galloylquinic acid derivatives and flavonoids are the main phenolic metabolites found in Copaifera langsdorffii leaves (Leguminosae, Detarioideae), a medicinal plant with potential therapeutic application in the treatment of kidney stones. The factors that affect metabolite production in this plant species are not well understood but may include environmental and genetic factors.
To quantify the variation in metabolite production over a 12-month period for 10 groups of C. langsdorffii cultivated under the same environmental conditions.
Copaifera langsdorffii seeds were collected from 10 different regions in southeast, Brazil and grown in the same field. HPLC-UV was used to quantify nine galloylquinic acid derivatives and two flavonoids in leaf samples from mature trees. Climate data for humidity, radiation, precipitation and temperature were provided by the National Institute of Meteorology, Brazil. Multivariate analyses were performed to correlate chemical and environmental variables.
The overall effect of environmental factors on the production of phenolic metabolites was uniform among C. langsdorffii groups. Chemical variation between groups was present, but small, and probably due to differences in their genetics and physiology. Seasonal changes influenced the production of the major phenolic metabolites, with increases in temperature and radiation levels favouring metabolite production.
When C. langsdorffii trees are cultivated in the same environment, the production of the major secondary metabolites found in their leaves is very similar quantitatively, varying based on geographic location of original population and seasonal changes. This favours the standardisation of plant raw material for the production of a phytomedicine.
奎尼酸衍生物和类黄酮是在药用植物巴西可可树（豆科，金合欢族）叶子中发现的主要酚类代谢物，具有治疗肾结石的潜在治疗应用。影响该植物物种代谢产物产生的因素尚不清楚，但可能包括环境和遗传因素。
定量比较 10 组在相同环境条件下种植的巴西可可树在 12 个月内代谢产物的产量变化。
从巴西东南部的 10 个不同地区收集巴西可可树种子，并在同一田间种植。HPLC-UV 用于定量测定成熟树木叶片样本中的 9 种奎尼酸衍生物和 2 种类黄酮。湿度、辐射、降水和温度的气候数据由巴西国家气象研究所提供。进行了多元分析以关联化学和环境变量。
环境因素对酚类代谢产物产生的总体影响在巴西可可树群体中是一致的。群体之间存在化学变异，但很小，可能是由于它们的遗传和生理差异。季节性变化影响主要酚类代谢产物的产生，温度和辐射水平的升高有利于代谢产物的产生。
当巴西可可树在相同的环境中种植时，其叶子中发现的主要次生代谢产物的产生在数量上非常相似，仅因原始种群的地理位置和季节性变化而有所不同。这有利于植物原料的标准化，以生产植物药。
