中国首次关于从患乳腺炎奶牛乳汁中分离出的象分枝杆菌鉴定及药敏情况的报告

First Report in China on the Identification and Drug Sensitivity of Mycobacterium elephantis Isolated from the Milk of a Cow with Mastitis.

作者信息

Ji Ling Yun, Xu Dong Lei, Yin Shu Peng, Liu Hai Can, Li Gui Lian, Jiang Yi, Wei Jian Hao, Zeng Hao, Lou Yong Liang, Lyu Jian Xin, Wan Kang Lin

机构信息

Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200123, China.

State Key Laboratory of Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention Beijing 102206, China.

出版信息

Biomed Environ Sci. 2017 Jul;30(7):501-507. doi: 10.3967/bes2017.066.

DOI:10.3967/bes2017.066

PMID:28756809

Abstract

OBJECTIVE

In this study, milk from a cow with mastitis was analyzed to determine the presence of mycobacterial infection. Milk quality and security problems pertaining to the safe consumption of dairy products were also discussed in this study.

METHODS

Milk was preprocessed with 4% NaOH. Then, mycobacteria were isolated from the milk sample on L-J medium. The isolate was identified using multiple loci Polymerase Chain Reaction (PCR) and multi-locus sequence analysis with 16S rRNA, sodA, hsp65, and ITS genes. The drug sensitivity of the isolate to 27 antibiotics was tested through alamar blue assay.

RESULTS

Smooth, moist, pale yellow colonies appeared on the L-J medium within a week after inoculation. Based on the results of multiple loci PCR analysis, the isolate was preliminarily identified as non-tuberculous mycobacteria. The 16S rRNA, SodA, hsp65, and ITS gene sequences of the isolate exhibited 99%, 99%, 99%, and 100% similarities, respectively, with those of the published reference strains of Mycobacterium elephantis (M. elephantis). The drug sensitivity results showed that the strain is resistant to isoniazid, p-aminosalicylic acid, and trimesulf but is sensitive to ofloxacin, rifampicin, amikacin, capreomycin, moxifloxacin, kanamycin, levofloxacin, cycloserine, ethambutol, streptomycin, tobramycin, rifabutin, ciprofloxacin, linezolid, cefoxitin, clarithromycin, and minocycline.

CONCLUSION

To the best of our knowledge, this study is initially to report the isolation of M. elephantis from the milk of a cow with mastitis in China.

摘要

目的

本研究对患有乳腺炎奶牛的乳汁进行分析，以确定是否存在分枝杆菌感染。本研究还讨论了与乳制品安全消费相关的牛奶质量和安全问题。

方法

牛奶用4%氢氧化钠进行预处理。然后，在罗-琴培养基上从牛奶样本中分离分枝杆菌。使用多位点聚合酶链反应（PCR）以及对16S rRNA、sodA、hsp65和ITS基因进行多位点序列分析来鉴定分离株。通过alamar蓝试验检测分离株对27种抗生素的药敏性。

结果

接种后一周内，罗-琴培养基上出现光滑、湿润、淡黄色菌落。基于多位点PCR分析结果，该分离株初步鉴定为非结核分枝杆菌。该分离株的16S rRNA、SodA、hsp65和ITS基因序列与已发表的象分枝杆菌（M. elephantis）参考菌株序列分别具有99%、99%、99%和100%的相似性。药敏结果显示，该菌株对异烟肼、对氨基水杨酸和复方磺胺甲恶唑耐药，但对氧氟沙星、利福平、阿米卡星、卷曲霉素、莫西沙星、卡那霉素、左氧氟沙星、环丝氨酸、乙胺丁醇、链霉素、妥布霉素、利福布汀、环丙沙星、利奈唑胺、头孢西丁、克拉霉素和米诺环素敏感。