Poor André P, Moreno Luisa Z, Matajira Carlos E C, Parra Beatriz M, Gomes Vasco T M, Silva Ana Paula S, Dutra Mauricio C, Christ Ana Paula G, Barbosa Mikaela R F, Sato Maria Inês Z, Moreno Andrea M
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo. Av Prof Dr Orlando Marques de Paiva, 87; Cidade Universitária. São Paulo/SP, Brazil.
Companhia Ambiental do Estado de São Paulo (CETESB). Av Prof Frederico Hermann Jr, 345; Pinheiros. São Paulo/SP, Brazil.
Vet Microbiol. 2017 Aug;207:149-152. doi: 10.1016/j.vetmic.2017.06.008. Epub 2017 Jun 15.
Porcine Corynebacterium infection is still poorly studied, even though the pig has been described as an asymptomatic carrier of Corynebacterium species, including the zoonotic species C. ulcerans, C. confusum and C. amycolatum. Here we present the identification, molecular and antimicrobial susceptibility characterization of coryneform bacteria isolated from sows with urinary tract infection. C. diphtheriae, C. confusum and C. amycolatum were isolated from sows with urinary infection and metritis. Corynebacterium species were identified by MALDI-TOF MS and confirmed by 16S rRNA and rpoB sequencing. All porcine C. diphtheriae strains were further characterized as non-toxigenic (tox). SE-AFLP genotyping was also performed and enabled not only Corynebacterium species differentiation but also the assessment of C. amycolatum genetic heterogeneity. All studied porcine Corynebacterium strains presented alarming resistance profiles with high MIC values for macrolides/lincosamide, tetracyclines and quinolones, which can be related with high usage in both veterinary and human medicine. Isolation of zoonotic Corynebacterium species from commercial swine is important for assessing the potential zoonotic risk for farmers and further implication for both human and animal treatment.
尽管猪被描述为棒状杆菌属的无症状携带者，包括人畜共患病种溃疡棒状杆菌、迷惑棒状杆菌和无枝菌酸棒状杆菌，但猪棒状杆菌感染仍研究不足。在此，我们展示了从患有尿路感染的母猪中分离出的棒状杆菌的鉴定、分子特征及抗菌药敏特性。从患有尿路感染和子宫炎的母猪中分离出了白喉棒状杆菌、迷惑棒状杆菌和无枝菌酸棒状杆菌。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定棒状杆菌属，并通过16S rRNA和rpoB测序进行确认。所有猪源白喉棒状杆菌菌株进一步被鉴定为无毒（tox）型。还进行了序列扩增多态性（SE-AFLP）基因分型，这不仅能够区分棒状杆菌属菌种，还能评估无枝菌酸棒状杆菌的遗传异质性。所有研究的猪源棒状杆菌菌株都呈现出令人担忧的耐药谱，对大环内酯类/林可酰胺类、四环素类和喹诺酮类药物的最低抑菌浓度（MIC）值很高，这可能与兽医和人类医学中的高用量有关。从商业猪中分离出人畜共患病的棒状杆菌菌种对于评估农民潜在的人畜共患病风险以及对人类和动物治疗的进一步影响具有重要意义。
