Program in Biosciences and Physiopathology, Universidade Estadual de Maringá (UEM), Av. Colombo 5790, Bloco T20, 87020-900 Maringá, PR, Brazil.
Program in Health Sciences, Universidade Estadual de Maringá (UEM), Av Colombo 5790, Bloco 126, 87020-900 Maringá, PR, Brazil..
Cytokine. 2017 Nov;99:240-248. doi: 10.1016/j.cyto.2017.07.016. Epub 2017 Jul 27.
To evaluate the effects of dynamized ethyl alcohol (Ethylicum)6cH and 30cH in mice infected with T. cruzi.
In a blind, randomized and controlled assay, 63 eight-week-old, Swiss, male mice, infected with IP (1400 trypomastigotes, T. cruzi-Y-strain), were allocated into groups: CNI-non-infected (n=12), CI-infected and non-treated (n=17), Et-infected, treated with Ethylicum 6cH (dilution 1:10) (n=17), Et-infected, treated with Ethylicum 30cH (dilution 1:10) (n=17). Treatment was administered 48h before and after infection, followed by 56h/56h periods, until the 9th day after infection (a.i), for 16 h. Survival and mortality were assessed until the 82nd day after infection (a.i.). TNF-α, IL-6, IL-10, IL-5 and IL-17A cytokines were assessed in serum (3-4 animals/group), at time T0 (before infection), T8 and T12 (8th and 12th a.i), using the Mouse Cytokine 20-Plex Panel Magnetic Kit (Invitrogen, USA). Inflammation was determined in heart sections (eosin-hematoxylin staining) and behavior was analyzed with ANY-maze® software. The study was approved by the Animal Ethics Committee/UEM. Statistica 8.0 and R 3.0.2 software were used for statistical analyses.
The greater survival observed in the Et group was related to decreased inflammation in heart tissue and increased IL-5 at T0 (p<0.05) and IL-10 at T8 (p<0.05), characterizing the Th2 response. It was also related to shorter periods of immobility, observed on day 12 a.i. The higher mortality in the Et group was related to increased inflammation in the heart and a higher concentration of IL-6 and TNF-α cytokines, characterizing the Th1 response.
The results demonstrate the beneficial effect of Ethylicum 6cH in acute murine infection by T. cruzi.
评估动态乙基酒精（Ethylicum）6cH 和 30cH 对感染 T. cruzi 的小鼠的影响。
在一项盲法、随机对照试验中，将 63 只 8 周龄、雄性、瑞士小鼠分为以下几组：CNI-未感染（n=12）、CI-感染且未治疗（n=17）、Et-感染、用 Ethylicum 6cH（稀释 1:10）治疗（n=17）、Et-感染、用 Ethylicum 30cH（稀释 1:10）治疗（n=17）。治疗于感染前和感染后 48 小时进行，随后进行 56 小时/56 小时的治疗，直至感染后第 9 天（a.i.），共 16 小时。直至感染后第 82 天（a.i.）评估存活和死亡情况。在血清中（每组 3-4 只动物），于 T0（感染前）、T8 和 T12（第 8 天和第 12 天 a.i.）时，使用 Mouse Cytokine 20-Plex Panel Magnetic Kit（Invitrogen，美国）评估 TNF-α、IL-6、IL-10、IL-5 和 IL-17A 细胞因子。使用伊红-苏木精染色评估心脏组织中的炎症情况，使用 ANY-maze®软件分析行为。该研究得到了 UEM 动物伦理委员会的批准。使用 Statistica 8.0 和 R 3.0.2 软件进行统计分析。
Et 组观察到的更高存活率与心脏组织中的炎症减轻有关，与 T0 时的 IL-5（p<0.05）和 T8 时的 IL-10（p<0.05）增加有关，这表明存在 Th2 反应。它还与感染后第 12 天观察到的更短的不动期有关。Et 组更高的死亡率与心脏炎症加重以及更高浓度的 IL-6 和 TNF-α 细胞因子有关，这表明存在 Th1 反应。
结果表明，Ethylicum 6cH 对 T. cruzi 急性感染的小鼠具有有益作用。
