Sandri Patrícia, Aleixo Denise Lessa, Sanchez Falkowski Gislaine Janaina, Nascimento Júnior Anélio Dias, Gomes Mônica Lúcia, Hernandes Luzmarina, Machado de Oliveira Dalalio Márcia, Moreira Neide Martins, Toledo Max Jean de Ornelas, Gabriel Maristela, de Araújo Silvana Marques
Universidade Estadual de Maringá, Departamento de Ciências Básicas da Saúde/Parasitologia, Maringá, PR, Brazil.
Universidade Estadual de Maringá, Departamento de Farmácia, Maringá, PR, Brazil.
Homeopathy. 2015 Jan;104(1):48-56. doi: 10.1016/j.homp.2014.05.007. Epub 2014 Jun 28.
This study evaluates the effect of Trypanosoma cruzi biotherapy 17dH (BIOT) on mice of different ages, infected with the protozoa concerned.
Performing a blind, controlled, randomized by drawing experiment, 110 animals four or eight-week-old, Swiss, male mice were divided into infected control treated hydroalcoholic 7% (CI-4 = 34 or CI-8 = 21 animals) and infected control treated with biotherapy 17dH-0.2 mL/animal/20 consecutive days/oral regimen (BIOT-4 = 33 or BIOT-8 = 21 animals). Animals were inoculated intraperitoneally with 1400 trypomastigote, T. cruzi Y-strain. Parasitological, immunological and histopathologic parameters were evaluated statistically, using Statistica-8.0 and R 3.0.2 program to analysis of survival. The study was approved by the Ethics Committee for Animal Experimentation/UEM.
Four-week-old mice showed no statistical difference in parasitemia (P = 0.5718) between the treated and control group. Eight-week-old mice from the treated group had a higher parasite peak (P = 0.0424) and higher parasitemia (P < 0.005) than the control. To both groups of 4 and 8 weeks of age, treated or untreated, survival of mice was higher in the treated group than in the control, although it was not statistically significant (p-value = 0.32, 0.55 respectively). Four-week-old mice displayed a spleen section with a number of amastigote nests significantly higher in BIOT-4 than CI-4 (P = 0.01). In eight-week-old mice the number of amastigote nests (P < 0.001) and inflammatory foci (P < 0.06-10% significance) in the liver section were smaller in BIOT-8 than CI-8. Spleen giant cells were significantly higher in CI-8 than in BIOT-8 (P < 0.01). Eight-week-old animals treated with biotherapy showed higher parasitemia and lower tissue parasitism. Opposite pattern was observed in four-week-old animals.
There is a difference of high diluted medication effect in four and eight-week-old mice. In the group of animals 8 weeks the immunomodulatory effect seems to have been higher. Hence, treatment with the medicine produced from T. cruzi modulates the inflammatory response with increased apoptosis and decreased serum levels of TGF-β.
本研究评估克氏锥虫生物疗法17dH（BIOT）对感染相关原生动物的不同年龄小鼠的影响。
进行一项盲法、对照、随机抽签实验，将110只4周龄或8周龄的瑞士雄性小鼠分为感染对照组，用7%水醇溶液处理（CI - 4 = 34只或CI - 8 = 21只动物）和感染对照组，用生物疗法17dH - 0.2 mL/动物/连续20天/口服给药方案处理（BIOT - 4 = 33只或BIOT - 8 = 21只动物）。动物腹腔注射1400个克氏锥虫Y株的锥鞭毛体。使用Statistica - 8.0和R 3.0.2程序对寄生虫学、免疫学和组织病理学参数进行统计学评估以分析生存率。该研究经动物实验伦理委员会/UEM批准。
4周龄小鼠治疗组和对照组之间的寄生虫血症无统计学差异（P = 0.5718）。治疗组的8周龄小鼠比对照组具有更高的寄生虫峰值（P = 0.0424）和更高的寄生虫血症（P < 0.005）。对于4周龄和8周龄的两组小鼠，无论是否治疗，治疗组小鼠的生存率均高于对照组，尽管差异无统计学意义（p值分别为0.32、0.55）。4周龄小鼠的脾脏切片中，BIOT - 4组的无鞭毛体巢数量显著高于CI - 4组（P = 0.01）。在8周龄小鼠中，BIOT - 8组肝脏切片中的无鞭毛体巢数量（P < 0.001）和炎症灶（P < 0.06 - 10%显著性）均少于CI - 8组。CI - 8组的脾脏巨细胞显著高于BIOT - 8组（P < 0.01）。接受生物疗法治疗的8周龄动物表现出更高的寄生虫血症和更低的组织寄生虫感染。在4周龄动物中观察到相反的模式。
4周龄和8周龄小鼠在高稀释药物效果方面存在差异。在8周龄的动物组中，免疫调节作用似乎更高。因此，用克氏锥虫制备的药物治疗可调节炎症反应，增加细胞凋亡并降低血清TGF -β水平。
