血清25-羟维生素D和1,25-二羟维生素D在预测终末期肾病和肾移植患者外周调节性T细胞和辅助性T细胞17计数方面的效用较低。
Low utility of serum 25-hydroxyvitamin D and 1, 25-dihydroxyvitamin D in predicting peripheral Treg and Th17 cell counts in ESRD and renal transplant patients.
作者信息
Aly Mostafa G, Zhu Li, Weimer Rolf, Opelz Gerhard, Morath Christian, Kuon Ruben, Tohamy Mohammed, Saadi Gamal, Soliman Mona, Ibrahim Walaa, Daniel Volker
机构信息
Transplantation-Immunology, Institute of Immunology, University Hospital Heidelberg, Im Neuenheimer Feld 305, 69120 Heidelberg, Germany; Nephrology Unit, Internal Medicine Department, Assiut University, Egypt; Department of Internal Medicine, University of Giessen, Klinikstrasse 33, D-35385 Giessen, Germany.
Transplantation-Immunology, Institute of Immunology, University Hospital Heidelberg, Im Neuenheimer Feld 305, 69120 Heidelberg, Germany; Department of Hematology, Tongji Hospital, Huazhong University of Science and Technology, 430030 Wuhan, China.
出版信息
Transpl Immunol. 2017 Aug;43-44:3-10. doi: 10.1016/j.trim.2017.07.003. Epub 2017 Jul 27.
BACKGROUND
Vitamin D has shown an immune-modulatory effect in different studies. Vitamin D stimulates Tregs and inhibits Th17 cells. The immune-modulatory role of vitamin D in chronic kidney disease (CKD) and renal transplant patients is unclear. We measured whether different serum levels of vitamin D were associated with an increased or decreased presence of lymphocyte subsets including Treg and Th17 cells in end-stage renal disease (ESRD) and renal transplant recipients.
METHODS
Eighty-seven renal transplant recipients and 53 end-stage renal disease (ESRD) patients were enrolled in this study. The absolute counts of CD4+ and CD8+ T, CD16+ CD56+ NK, CD19+ B, CD4+ CD25+ CD127- Foxp3+ (Tregs), Helios+ Tregs, CD38+ Tregs, and CD4+ CD17+ (Th17) cells were analyzed in peripheral blood in both patient groups. In addition, serum 25 (OH) D, 1, 25 (OH) D, IL-6, IL-17, IL-23, and TGF-β were measured. The association between lymphocyte subset counts and 1, 25 (OH) D or 25 (OH) D was studied, as was the association between serum IL-6, IL-17, IL-23, or TGF-β and 1,25 (OH) D or 25 (OH) D.
RESULTS
Serum 25 (OH) D and 1,25 (OH) D levels were not independently associated with peripheral CD4+ T, CD19+ B, CD16+ CD56+ NK, Treg, or Th17 cell counts. In contrast to serum 25 (OH) D, serum1, 25 (OH) D was positively associated with CD8+ T cells counts in renal transplant recipients.
CONCLUSION
Our findings indicate low utility of serum 25 (OH) D and 1, 25 (OH) D levels in predicting a change in lymphocyte subset counts in ESRD and renal transplant patients.
背景
维生素D在不同研究中显示出免疫调节作用。维生素D可刺激调节性T细胞（Tregs）并抑制辅助性T细胞17（Th17）细胞。维生素D在慢性肾脏病（CKD）和肾移植患者中的免疫调节作用尚不清楚。我们测定了不同血清水平的维生素D是否与终末期肾病（ESRD）和肾移植受者中包括Tregs和Th17细胞在内的淋巴细胞亚群数量的增加或减少有关。
方法
本研究纳入了87名肾移植受者和53名终末期肾病（ESRD）患者。分析了两组患者外周血中CD4⁺和CD8⁺T细胞、CD16⁺CD56⁺自然杀伤（NK）细胞、CD19⁺B细胞、CD4⁺CD25⁺CD127⁻叉头框蛋白3⁺（Tregs）、太阳神（Helios）⁺Tregs、CD38⁺Tregs和CD4⁺CD17⁺（Th17）细胞的绝对计数。此外，还测定了血清25（OH）D、1,25（OH）D、白细胞介素-6（IL-6）、IL-17、IL-23和转化生长因子-β（TGF-β）。研究了淋巴细胞亚群计数与1,25（OH）D或25（OH）D之间的关联，以及血清IL-6、IL-17、IL-23或TGF-β与1,25（OH）D或25（OH）D之间的关联。
结果
血清25（OH）D和1,25（OH）D水平与外周血CD4⁺T细胞、CD19⁺B细胞、CD16⁺CD56⁺NK细胞、Tregs或Th17细胞计数无独立相关性。与血清25（OH）D相反，血清1,25（OH）D与肾移植受者的CD8⁺T细胞计数呈正相关。
结论
我们的研究结果表明，血清25（OH）D和1,25（OH）D水平在预测ESRD和肾移植患者淋巴细胞亚群计数变化方面作用不大。
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