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使用器官浴从分离的大鼠胰腺制剂中实现可重复的胰岛素分泌。

Reproducible insulin secretion from isolated rat pancreas preparations using an organ bath.

作者信息

Morita Asuka, Ouchi Motoshi, Terada Misao, Kon Hiroe, Kishimoto Satoko, Satoh Keitaro, Otani Naoyuki, Hayashi Keitaro, Fujita Tomoe, Inoue Ken-Ichi, Anzai Naohiko

机构信息

Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine, 880 Kitakobayashi, Mibu, Shimotsuga, Tochigi 321-0293, Japan.

Laboratory Animal Research Center, Dokkyo Medical University, 880 Kitakobayashi, Mibu, Shimotsuga, Tochigi 321-0293, Japan.

出版信息

Exp Anim. 2018 Feb 9;67(1):15-22. doi: 10.1538/expanim.17-0059. Epub 2017 Jul 28.

Abstract

Diabetes mellitus is a lifestyle-related disease that is characterized by inappropriate or diminished insulin secretion. Ex vivo pharmacological studies of hypoglycemic agents are often conducted using perfused pancreatic preparations. Pancreas preparations for organ bath experiments do not require cannulation and are therefore less complex than isolated perfused pancreas preparations. However, previous research has generated almost no data on insulin secretion from pancreas preparations using organ bath preparations. The purpose of this study was to investigate the applicability of isolated rat pancreas preparations using the organ bath technique in the quantitative analysis of insulin secretion from β-cells. We found that insulin secretion significantly declined during incubation in the organ bath, whereas it was maintained in the presence of 1 µM GLP-1. Conversely, amylase secretion exhibited a modest increase during incubation and was not altered in the presence of GLP-1. These results demonstrate that the pancreatic organ bath preparation is a sensitive and reproducible method for the ex vivo assessment of the pharmacological properties of hypoglycemic agents.

摘要

糖尿病是一种与生活方式相关的疾病,其特征是胰岛素分泌不当或减少。降血糖药物的体外药理学研究通常使用灌注胰腺制剂进行。用于器官浴实验的胰腺制剂不需要插管,因此比分离的灌注胰腺制剂更简单。然而,以前的研究几乎没有产生关于使用器官浴制剂的胰腺制剂胰岛素分泌的数据。本研究的目的是探讨使用器官浴技术的分离大鼠胰腺制剂在定量分析β细胞胰岛素分泌中的适用性。我们发现,在器官浴孵育期间胰岛素分泌显著下降,而在存在1µM胰高血糖素样肽-1(GLP-1)的情况下胰岛素分泌得以维持。相反,淀粉酶分泌在孵育期间有适度增加,并且在存在GLP-1的情况下没有改变。这些结果表明,胰腺器官浴制剂是一种用于体外评估降血糖药物药理学特性的灵敏且可重复的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e4/5814310/d57df4deafe9/expanim-67-015-g001.jpg

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