Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine, 880 Kitakobayashi, Mibu, Shimotsuga, Tochigi 321-0293, Japan.
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine, 880 Kitakobayashi, Mibu, Shimotsuga, Tochigi 321-0293, Japan.
J Pharmacol Sci. 2022 Jun;149(2):66-72. doi: 10.1016/j.jphs.2022.03.005. Epub 2022 Apr 4.
Organ bath experiments are conventionally used to investigate the physiological actions and effects of hormones and drugs on organ responses. We developed an experimental method to reproduce insulin secretion from isolated rat pancreas preparations, to investigate substances that promote insulin secretion ex vivo. 1,5-anhydro-D-glucitol (1,5-AG) is found in foods, and exists in humans and rodents; however, whether 1,5-AG stimulates insulin secretion remains unclear. This study aimed to assess the effects of short-term 1,5-AG stimulation on insulin secretion in both ex vivo and in INS-1E (rat-derived) cells in vitro. Our results indicated that 1,5-AG had no potency to increase the proportion of insulin outflow both in ex vivo and in vitro experiments. Insulin outflow significantly increased upon stimulation with 10 μM glimepiride, a member of the sulfonylurea class of drugs, ex vivo. Glucose-stimulated insulin secretion was observed not only in INS-1E cells but also in rat pancreatic preparations. Our findings demonstrated that short-term exposure to 1,5-AG had no effect on insulin secretion in rats.
器官浴实验通常用于研究激素和药物对器官反应的生理作用和影响。我们开发了一种实验方法来重现分离的大鼠胰腺制剂中的胰岛素分泌，以研究促进胰岛素分泌的物质。1,5-脱水葡萄糖（1,5-AG）存在于食物中，在人类和啮齿动物中都存在；然而，1,5-AG 是否刺激胰岛素分泌尚不清楚。本研究旨在评估短期 1,5-AG 刺激对离体和体外 INS-1E（大鼠衍生）细胞中胰岛素分泌的影响。我们的结果表明，1,5-AG 对离体和体外实验中胰岛素外流的比例均没有促进作用。10 μM 格列美脲（磺酰脲类药物的一种）刺激离体时，胰岛素外流显著增加。葡萄糖刺激的胰岛素分泌不仅在 INS-1E 细胞中观察到，在大鼠胰腺制剂中也观察到。我们的发现表明，短期暴露于 1,5-AG 对大鼠的胰岛素分泌没有影响。
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