Al-Qaysi Safaa A S, Al-Haideri Halah, Thabit Zaid Akram, Al-Kubaisy Wijdan Hameed Abd Al-Razzaq, Ibrahim Jamal Abd Al-Rahman
Department of Biology, College of Science for Women, University of Baghdad, Baghdad, Iraq.
Environmental Biotechnology Department, Biotechnology Research Center, Al Nahrain University, Baghdad, Iraq.
Int J Microbiol. 2017;2017:2605382. doi: 10.1155/2017/2605382. Epub 2017 Jul 3.
The present study was conducted to estimate the antimicrobial activity and the potential biological control of the killer toxin produced by DSMZ70238 against several pathogenic microorganisms. In this study, the effects of NaCl, pH, and temperature, killer toxin production, and antimicrobial activity were studied. The results showed that the optimum inhibitory effect of killer toxin was at 8% NaCl, and the diameters of clear zones were 20, 22, 22, 21, 14, and 13 mm for and , respectively. The largest inhibition zones were observed at pH 4.5 with inhibition zone of 16, 18, 17, 18, 11, and 12 mm for the same microorganisms. The results also showed that 25°C is the optimal temperature for toxin killing activity against all targeted microorganisms. In addition, the activity of killer toxin significantly inhibited the growth of fungal mycelia for all target pathogenic fungi and the percentages of inhibition were 47.77, 48.88, 52.22, and 61.11% for and , respectively. The results showed the highest growth rate of DSMZ70238 under condition of 8% NaCl concentration, pH 4.5, and 25°C for 72 h.
本研究旨在评估DSMZ70238产生的杀伤毒素对几种致病微生物的抗菌活性及潜在生物防治作用。在本研究中，研究了NaCl、pH值和温度对杀伤毒素产生及抗菌活性的影响。结果表明，杀伤毒素的最佳抑制效果出现在8% NaCl浓度下，对于[具体微生物名称1]、[具体微生物名称2]等，透明圈直径分别为20、22、22、21、14和13毫米。在pH 4.5时观察到最大抑制圈，对于相同微生物，抑制圈直径为16、18、17、18、11和12毫米。结果还表明，25°C是杀伤毒素对所有目标微生物发挥杀伤活性的最佳温度。此外，杀伤毒素的活性显著抑制了所有目标致病真菌的真菌菌丝体生长，对于[具体真菌名称1]、[具体真菌名称2]等，抑制率分别为47.77%、48.88%、52.22%和61.11%。结果表明，DSMZ70238在8% NaCl浓度、pH 4.5和25°C条件下培养72小时时生长速率最高。
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