Hicks Robert H, Moreno-Beltrán Mauro, Gore-Lloyd Deborah, Chuck Christopher J, Henk Daniel A
Milner Centre for Evolution, University of Bath, Bath BA2 7AY, UK.
Department of Chemical Engineering, University of Bath, Bath BA2 7AY, UK.
Biology (Basel). 2021 Nov 24;10(12):1227. doi: 10.3390/biology10121227.
is a non-conventional yeast with potential to be used in biotechnological processes, especially those involving low-cost feedstock exploitation and biocontrol applications. The combination of traits that supports these industrial applications in also makes it an attractive option to study in the context of livestock health. In this study, we examined the specific interactions between and multiple avian pathogenic bacteria. We tested individual bacteria-yeast interactions and bacterial combinations in both solid and liquid media and in variable nutrient environments. Across multiple isolates of , we observed different levels of antimicrobial activity, varying from supporting the growth of competing bacteria through suppression and bacterial killing, and we found that these responses varied depending on the bacterial strains and media. We identified multiple molecular routes, including proteins produced by strains, that acted to control these microbial interactions. Furthermore, protein screening revealed that strains were induced to produce proteins specifically when exposed to bacterial strains, suggesting that fine-tuned mechanisms allow to function as a potential lynchpin in a microbial community.
是一种非常规酵母，有潜力用于生物技术过程，特别是那些涉及低成本原料开发和生物防治应用的过程。支持这些工业应用的特性组合也使其成为在牲畜健康背景下进行研究的有吸引力的选择。在本研究中，我们研究了与多种禽病原菌之间的具体相互作用。我们在固体和液体培养基以及可变营养环境中测试了单个细菌与酵母的相互作用以及细菌组合。在多个分离株中，我们观察到不同水平的抗菌活性，从支持竞争细菌的生长到抑制和杀灭细菌，并且我们发现这些反应因细菌菌株和培养基而异。我们确定了多种分子途径，包括菌株产生的蛋白质，这些途径起到控制这些微生物相互作用的作用。此外，蛋白质筛选显示，菌株在暴露于细菌菌株时会被诱导特异性产生蛋白质，这表明微调机制使能够作为微生物群落中的潜在关键因素发挥作用。
