Wu Qin, Cheng Qinqin, Yuan Siming, Qian Junchao, Zhong Kai, Qian Yinfeng, Liu Yangzhong
CAS Key Laboratory of Soft Matter Chemistry , CAS , High Magnetic Field Laboratory , Department of Chemistry , University of Science and Technology of China , Hefei , 230026 , Anhui , China . Email:
High Magnetic Field Laboratory , Chinese Academy of Sciences , Hefei , 230031 , China.
Chem Sci. 2015 Nov 1;6(11):6607-6613. doi: 10.1039/c5sc01925g. Epub 2015 Aug 11.
Multimodal imaging is a highly desirable biomedical application since it can provide complementary information from each imaging modality. We propose a protein engineering-based strategy for the construction of a bimodal probe for fluorescence and magnetic resonance imaging. A recombinant protein was generated by the fusion of a supercharged green fluorescence protein (GFP) with a lanthanide-binding tag (dLBT) that can stably bind two Gd ions. The GFP-dLBT fusion protein showed strong fluorescence and exhibited efficient contrast enhancement in magnetic resonance imaging. This protein probe improves the MR relaxation more efficiently than Gd-DTPA (gadopentetate dimeglumine). The superior cell-penetrating activity of GFP allows the efficient cellular uptake of this fusion protein and it can thus be used as a cellular imaging probe. Dual imaging was conducted and in mice. This result indicates that the fusion of different functional domains is a feasible approach for making multi-modal imaging agents.
多模态成像因其能够从每种成像模态提供互补信息，是一种非常理想的生物医学应用。我们提出了一种基于蛋白质工程的策略，用于构建用于荧光和磁共振成像的双模态探针。通过将超荷绿色荧光蛋白（GFP）与可稳定结合两个钆离子的镧系元素结合标签（dLBT）融合，生成了一种重组蛋白。GFP-dLBT融合蛋白显示出强荧光，并在磁共振成像中表现出有效的对比度增强。这种蛋白质探针比钆喷酸葡胺（Gd-DTPA）更有效地改善了磁共振弛豫。GFP卓越的细胞穿透活性使这种融合蛋白能够有效地被细胞摄取，因此它可以用作细胞成像探针。在小鼠体内进行了双重成像。这一结果表明，融合不同的功能域是制备多模态成像剂的一种可行方法。
