Yoo Jason J, Kim Joohoon, Crooks Richard M
Department of Chemistry , The Center for Nano- and Molecular Science and Technology , The University of Texas at Austin , 105 E. 24th St. Stop A5300 , Austin , TX 78712-1224 , USA . Email:
Department of Chemistry , Research Institute for Basic Sciences , Kyung Hee University , Seoul 130-701 , South Korea.
Chem Sci. 2015 Nov 1;6(11):6665-6671. doi: 10.1039/c5sc02259b. Epub 2015 Jul 29.
Here, we report on the electrochemical detection of individual collisions between a conjugate consisting of silver nanoparticles (AgNPs) linked to conductive magnetic microbeads (cMμBs) DNA hybridization and a magnetized electrode. The important result is that the presence of the magnetic field increases the flux of the conjugate to the electrode surface, and this in turn increases the collision frequency and improves the limit of detection (20 aM). In addition, the magnitude of the charge associated with the collisions is greatly enhanced in the presence of the magnetic field. The integration of DNA into the detection protocol potentially provides a means for using electrochemical collisions for applications in biological and chemical sensing.
在此，我们报告了一种共轭物（由连接到导电磁性微珠（cMμBs）的银纳米颗粒（AgNPs）组成）与磁化电极之间单个碰撞的电化学检测。重要的结果是，磁场的存在增加了共轭物到达电极表面的通量，这反过来又增加了碰撞频率并提高了检测限（20 aM）。此外，在磁场存在的情况下，与碰撞相关的电荷量大大增加。将DNA整合到检测方案中可能为将电化学碰撞用于生物和化学传感应用提供一种手段。
