“埃及丝瓜植原体（暂定种）”，一种与埃及丝瓜（Luffa aegyptica Mill.）的扫帚病相关的新分类单元。
'Candidatus Phytoplasma luffae', a novel taxon associated with witches' broom disease of loofah, Luffa aegyptica Mill.
作者信息
Davis Robert E, Zhao Yan, Wei Wei, Dally Ellen L, Lee Ing-Ming
机构信息
Molecular Plant Pathology Laboratory, USDA-Agricultural Research Service, Beltsville, MD 20705, USA.
出版信息
Int J Syst Evol Microbiol. 2017 Aug;67(8):3127-3133. doi: 10.1099/ijsem.0.001980. Epub 2017 Jul 31.
The phytoplasma associated with witches' broom disease of loofah [Luffa aegyptica Mill., syn. Luffa cylindrica (L.) M.J. Roem.] in Taiwan was classified in group 16SrVIII, subgroup A (16SrVIII-A), based on results from actual and in silico RFLP analysis of 16S rRNA gene sequences. Nucleotide sequencing of PCR-amplified, cloned DNA segments revealed rrn interoperon sequence heterogeneity in the loofah witches' broom (LfWB) phytoplasma. Whereas the 16S-23S rRNA spacer region of rrnA contained a complete tRNA-Ile gene, the spacer of rrnB contained a nonfunctional remnant of a tRNA gene. Phylogenetic analysis of the rrnA and rrnB 16S rRNA genes revealed that the LfWB phytoplasma represented a distinct lineage within the phytoplasma clade, and the LfWB phytoplasma shared less than 97.5 % nucleotide sequence similarity of 16S rRNA genes with previously described 'CandidatusPhytoplasma' taxa. Based on unique properties of DNA, we propose recognition of loofah witches' broom phytoplasma strain LfWBR as representative of a novel taxon, 'CandidatusPhytoplasma luffae'.
基于对16S rRNA基因序列的实际和电子RFLP分析结果，与台湾丝瓜[埃及丝瓜（Luffa aegyptica Mill.，同物异名：圆筒丝瓜（Luffa cylindrica (L.) M.J. Roem.）]扫帚病相关的植原体被归类为16SrVIII组A亚组（16SrVIII-A）。对PCR扩增、克隆的DNA片段进行核苷酸测序，揭示了丝瓜扫帚病（LfWB）植原体中rrn操纵子间序列的异质性。rrnA的16S-23S rRNA间隔区包含一个完整的tRNA-Ile基因，而rrnB的间隔区包含一个tRNA基因的无功能残余片段。对rrnA和rrnB的16S rRNA基因进行系统发育分析表明，LfWB植原体代表了植原体进化枝内一个独特的谱系，且LfWB植原体与先前描述的“候选植原体”分类群的16S rRNA基因核苷酸序列相似性低于97.5%。基于DNA的独特特性，我们建议将丝瓜扫帚病植原体菌株LfWBR认定为一个新分类单元“候选丝瓜植原体（CandidatusPhytoplasma luffae）”的代表。
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