Rodrigues Jardim Bianca, Kinoti Wycliff M, Tran-Nguyen Lucy T T, Gambley Cherie, Rodoni Brendan, Constable Fiona E
School of Applied Systems Biology, La Trobe University, Bundoora, Victoria, Australia.
Agriculture Victoria Research, Department of Jobs, Precincts and Regions, AgriBio, Bundoora, Australia.
Int J Syst Evol Microbiol. 2021 Jan;71(1). doi: 10.1099/ijsem.0.004589. Epub 2020 Dec 8.
In Australia, little leaf (StLL) phytoplasma has been detected in Vogel, Krapov, L., L., L., and L. The 16S rRNA gene sequence of StLL phytoplasma strains from , and were compared and share 99.93-100 % nucleotide sequence identity. Phylogenetic comparisons between the 16S rRNA genes of StLL phytoplasma and other ' Phytoplasma' species indicate that StLL represents a distinct phytoplasma lineage. It shares its most recent known ancestry with '. Phytoplasma luffae' (16SrVIII-A), with which it has 97.17-97.25 % nucleotide identity. RFLP analysis of the 16S rRNA amplicon using PhyClassifier indicate that StLL phytoplasmas have a unique pattern (similarity coefficient below 0.85) that is most similar to that of . Phytoplasma luffae'. The unique RFLP patterns were confirmed . Nucleotide sequences of genes that are more variable than the 16S rRNA gene, namely (tu-elongation factor), (partial translocation gene), and the partial () gene operon (), produced phylogenetic trees with similar branching patterns to the 16S rRNA gene tree. Sequence comparisons between the StLL 16S rRNA spacer region confirmed previous reports of interoperon sequence heterogeneity for StLL, where the spacer region of encodes a complete tRNA-Isoleucine gene and the region does not. Together these results suggest that the Australian phytoplasma, StLL, is unique according to the International Organization for Mycoplasmology (IRPCM) recommendations. The novel taxon '. Phytoplasma stylosanthis' is proposed, with the most recent strain from a potato crop in Victoria, Australia, serving as the reference strain (deposited in the Victorian Plant Pathology Herbarium as VPRI 43683).
在澳大利亚，已在沃格尔、克拉波夫、L.、L.、L.和L.中检测到小叶（StLL）植原体。对来自[具体地点未明确]、[具体地点未明确]和[具体地点未明确]的StLL植原体菌株的16S rRNA基因序列进行了比较，核苷酸序列同一性为99.93 - 100%。StLL植原体与其他“植原体”物种的16S rRNA基因的系统发育比较表明，StLL代表一个独特的植原体谱系。它与“丝瓜植原体”（16SrVIII - A）拥有最近的已知共同祖先，与其核苷酸同一性为97.17 - 97.25%。使用PhyClassifier对16S rRNA扩增子进行的RFLP分析表明，StLL植原体具有独特的模式（相似系数低于0.85），与“丝瓜植原体”的模式最为相似。独特的RFLP模式得到了证实。比16S rRNA基因更具变异性的基因，即延伸因子（tu - elongation factor）、部分易位基因（partial translocation gene）和部分核糖体蛋白（ribosomal protein）基因操纵子（operon）的核苷酸序列，产生了与16S rRNA基因树具有相似分支模式的系统发育树。StLL 16S rRNA间隔区的序列比较证实了之前关于StLL操纵子间序列异质性的报道，其中[具体菌株未明确]的间隔区编码一个完整的异亮氨酸tRNA基因，而[具体菌株未明确]区域则不编码。这些结果共同表明，根据国际支原体学组织（IRPCM）的建议，澳大利亚的StLL植原体是独特的。提出了新分类单元“Stylosanthis植原体”，来自澳大利亚维多利亚州马铃薯作物的最新菌株作为参考菌株（保藏于维多利亚植物病理学标本馆，编号为VPRI 43683）。
