Dong Shuang, Wang Jian-Ming, Cheng Li-Min, Lu Yan-Li, Li Shu-Hong, Chen Ye
Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology , Tianjin 300457, China.
J Agric Food Chem. 2017 Aug 30;65(34):7352-7360. doi: 10.1021/acs.jafc.7b02205. Epub 2017 Aug 17.
The effects of atmospheric cold plasma (ACP) on zein in aqueous ethanol (80%, v/v) were investigated including particle size distribution, molecular structure, and content of free sulfhydryl (free-SH) group and disulfide bond, etc. The film-forming properties of zein films were also characterized. After ACP treatment, the particle size of zein aggregates showed a remarkable decrease and uniform particle distribution. There was a downward trend both in pH value and viscosity with the increasing ACP treatment intensity. Moreover, the increase of disulfide bonds concentration was suggested to be correlated to the compact structure strengthened by cross-linking between zein molecules. It was proved from SEM micrographs that plasma could significantly decrease the aggregation degree of zein micelles. There was a slight decrease of the peak intensity in UV and fluorescence spectra compared with native zein, indicating the bulk structure of zein solution had not been disrupted. The reinforced flexibility and tensile strength of zein films had been observed after treatment on film-forming solution. This study provided an experimental basis for the investigation on behavior of plasma-treated protein in solution.
研究了常压冷等离子体（ACP）对乙醇水溶液（80%，v/v）中玉米醇溶蛋白的影响，包括粒径分布、分子结构、游离巯基（free-SH）基团和二硫键含量等。还对玉米醇溶蛋白膜的成膜性能进行了表征。经ACP处理后，玉米醇溶蛋白聚集体的粒径显著减小，颗粒分布均匀。随着ACP处理强度的增加，pH值和粘度均呈下降趋势。此外，二硫键浓度的增加被认为与玉米醇溶蛋白分子间交联增强的紧密结构有关。扫描电子显微镜照片证明，等离子体可显著降低玉米醇溶蛋白胶束的聚集程度。与天然玉米醇溶蛋白相比，紫外光谱和荧光光谱的峰强度略有下降，表明玉米醇溶蛋白溶液的整体结构未被破坏。对成膜溶液进行处理后，观察到玉米醇溶蛋白膜的柔韧性和拉伸强度增强。该研究为等离子体处理蛋白质在溶液中的行为研究提供了实验依据。
